Co-infecting pathogen lineages have additive effects on host bacterial communities

Autor(es)

Medina, Daniel ^{[1, 2]} ; Greenspan, Sasha E. ^[3] ; Carvalho, Tamilie ^[1] ; Becker, C. Guilherme ^[3] ; Toledo, Luis Felipe ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Lab Hist Nat Anfibios Brasileiros LaHNAB, R Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

^[2] SENACYT, Sistema Nacl Invest, Bldg 205, Clayton - Panama

^[3] Univ Alabama, Dept Biol Sci, 1339 Sci & Engn Complex, Tuscaloosa, AL 35487 - USA

• Tipo de documento: Artigo Científico
• Fonte: FEMS MICROBIOLOGY ECOLOGY; v. 97, n. 4 APR 2021.
• Citações Web of Science: 0

Resumo

Amphibian skin bacteria may confer protection against the fungus Batrachochytrium dendrobatidis (Bd), but responses of skin bacteria to different Bd lineages are poorly understood. The global panzootic lineage (Bd-GPL) has caused amphibian declines and extinctions globally. However, other lineages are enzootic (Bd-Asia-2/Brazil). Increased contact rates between Bd-GPL and enzootic lineages via globalization pose unknown consequences for host-microbiome-pathogen dynamics. We conducted a laboratory experiment and used 16S rRNA amplicon-sequencing to assess: (i) whether two lineages (Bd-Asia-2/Brazil and Bd-GPL) and their recombinant, in single and mixed infections, differentially affect amphibian skin bacteria; (ii) and the changes associated with the transition to laboratory conditions. We determined no clear differences in bacterial diversity among Bd treatments, despite differences in infection intensity. However, we observed an additive effect of mixed infections on bacterial alpha diversity and a potentially antagonistic interaction between Bd genotypes. Additionally, observed changes in community composition suggest a higher ability of Bd-GPL to alter skin bacteria. Lastly, we observed a drastic reduction in bacterial diversity and a change in community structure in laboratory conditions. We provide evidence for complex interactions between Bd genotypes and amphibian skin bacteria during coinfections, and expand on the implications of experimental conditions in ecological studies. (AU)

• Processo FAPESP: 19/18335-5 - Transporte aéreo passivo de um patógeno letal para anfíbios em áreas de elevadas: aplicações práticas para conservação de UCs do estado de São Paulo
• Beneficiário: Luis Felipe de Toledo Ramos Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 18/08650-8 - Microbioma anfíbio: a primeira linha de defesa contra a infecção pelo quitrídio
• Beneficiário: Daniel Christofer Medina Lopez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/25358-3 - O fungo quitrídio no Brasil: da sua origem às suas consequências
• Beneficiário: Luis Felipe de Toledo Ramos Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático