| Full text | |
| Author(s): |
Medina, Daniel
[1, 2]
;
Greenspan, Sasha E.
[3]
;
Carvalho, Tamilie
[1]
;
Becker, C. Guilherme
[3]
;
Toledo, Luis Felipe
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Biol, Lab Hist Nat Anfibios Brasileiros LaHNAB, R Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] SENACYT, Sistema Nacl Invest, Bldg 205, Clayton - Panama
[3] Univ Alabama, Dept Biol Sci, 1339 Sci & Engn Complex, Tuscaloosa, AL 35487 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | FEMS MICROBIOLOGY ECOLOGY; v. 97, n. 4 APR 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Amphibian skin bacteria may confer protection against the fungus Batrachochytrium dendrobatidis (Bd), but responses of skin bacteria to different Bd lineages are poorly understood. The global panzootic lineage (Bd-GPL) has caused amphibian declines and extinctions globally. However, other lineages are enzootic (Bd-Asia-2/Brazil). Increased contact rates between Bd-GPL and enzootic lineages via globalization pose unknown consequences for host-microbiome-pathogen dynamics. We conducted a laboratory experiment and used 16S rRNA amplicon-sequencing to assess: (i) whether two lineages (Bd-Asia-2/Brazil and Bd-GPL) and their recombinant, in single and mixed infections, differentially affect amphibian skin bacteria; (ii) and the changes associated with the transition to laboratory conditions. We determined no clear differences in bacterial diversity among Bd treatments, despite differences in infection intensity. However, we observed an additive effect of mixed infections on bacterial alpha diversity and a potentially antagonistic interaction between Bd genotypes. Additionally, observed changes in community composition suggest a higher ability of Bd-GPL to alter skin bacteria. Lastly, we observed a drastic reduction in bacterial diversity and a change in community structure in laboratory conditions. We provide evidence for complex interactions between Bd genotypes and amphibian skin bacteria during coinfections, and expand on the implications of experimental conditions in ecological studies. (AU) | |
| FAPESP's process: | 19/18335-5 - Passive air transportation of an amphibian lethal pathogen in high elevation sites: practical applications for protected areas conservation in the State of São Paulo |
| Grantee: | Luis Felipe de Toledo Ramos Pereira |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 18/08650-8 - Amphibian microbiome: the first line of defense against chytrid infection |
| Grantee: | Daniel Christofer Medina Lopez |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/25358-3 - The chytrid fungus: from its origins to its consequences |
| Grantee: | Luis Felipe de Toledo Ramos Pereira |
| Support Opportunities: | Research Projects - Thematic Grants |