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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bone demineralization improves onlay graft consolidation: A histological study in rat calvaria

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Autor(es):
Carvalho, Erika B. S. [1] ; Veronesi, Giovana F. [1] ; Manfredi, Gustavo G. P. [1] ; Damante, Carla A. [1] ; Sant'Ana, Adriana C. P. [1] ; Greghi, Sebastiao L. A. [1] ; Zangrando, Mariana S. R. [1] ; Consolaro, Alberto [2] ; Rezende, Maria L. R. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Div Pathol, Dept Stomatol, Bauru, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Periodontology; v. 92, n. 6, p. 1-10, JUN 2021.
Citações Web of Science: 0
Resumo

Background Previous data suggest that bone demineralization may promote bone graft consolidation as well as proliferation and differentiation of pre-osteoblasts, but the biological mechanisms involved in this process need to be clarified. This study investigated the effects of bone demineralization with citric acid (CA) and tetracycline (TCN) on the repair of onlay bone grafts. Methods Onlay bone grafts were performed on the calvaria of 126 Wistar rats. The contacting surfaces between bone graft and receptor bone bed were demineralized for 15, 30, and 60 seconds with TCN (50 mg/mL), or 10% CA, (pH 1), constituting the following test groups (n = 18): TCN15, TCN30, TCN60, CA15, CA30, and CA60. Control grafts (C) were performed without demineralization (n = 18). After 7, 30, and 60 days, biopsies were obtained for quantitative and qualitative histological analysis (a = 6). Results Demineralization accelerated the bone repair early from 7 days of healing. Higher percentage area of newly formed bone was observed in CA15 and TCN60 groups when compared to C in all evaluation periods (P = 0.02). At 30 days, C specimens had lower percentage of consolidated surfaces than TCN60, TCN30 and CA15 (P = 0.0015). At 60 days, CA15, CA60, and TCN60 presented bone surfaces almost completely filled by newly formed bone, against about 75% in C specimens (P = 0.0015). Conclusions Both CA and TCN were effective in accelerating osteogenesis at the interface between bone grafts and receptor bone beds, especially when applied for 15 seconds and 60 seconds, respectively. (AU)

Processo FAPESP: 14/15136-8 - Efeito da desmineralização óssea nos processos de reparo tecidual: estudo microscópico, estrutural e da atividade de células osteoblásticas em calvária de ratos
Beneficiário:Maria Lúcia Rubo de Rezende
Linha de fomento: Auxílio à Pesquisa - Regular