The B Chromosomes of Prochilodus lineatus (Teleostei, Characiformes) Are Highly Enriched in Satellite DNAs

Autor(es)

Stornioli, Jose Henrique Forte ^[1] ; Goes, Caio Augusto Gomes ^[1] ; Calegari, Rodrigo Milan ^[1] ; dos Santos, Rodrigo Zeni ^[1] ; Giglio, Leonardo Moura ^[1] ; Foresti, Fausto ^[2] ; Oliveira, Claudio ^[2] ; Penitente, Manolo ^[3] ; Porto-Foresti, Fabio ^[1] ; Utsunomia, Ricardo ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Fac Sci, BR-17033360 Bauru, SP - Brazil

^[2] Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, BR-18618689 Botucatu, SP - Brazil

^[3] Mato Grosso State Univ, BR-78400000 Cuiaba, MG - Brazil

^[4] Rural Fed Univ Rio De Janeiro, Inst Biol Sci & Hlth, Dept Genet, BR-23897000 Seropedica, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 10, n. 6 JUN 2021.
• Citações Web of Science: 0

Resumo

B or supernumerary chromosomes are dispensable elements that are widely present in numerous eukaryotes. Due to their non-recombining nature, there is an evident tendency for repetitive DNA accumulation in these elements. Thus, satellite DNA plays an important role in the evolution and diversification of B chromosomes and can provide clues regarding their origin. The characiform Prochilodus lineatus was one of the first discovered fish species bearing B chromosomes, with all populations analyzed so far showing one to nine micro-B chromosomes and exhibiting at least three morphological variants (Ba, Bsm, and Bm). To date, a single satellite DNA is known to be located on the B chromosomes of this species, but no information regarding the differentiation of the proposed B-types is available. Here, we characterized the satellitome of P. lineatus and mapped 35 satellite DNAs against the chromosomes of P. lineatus, of which six were equally located on all B-types and this indicates a similar genomic content. In addition, we describe, for the first time, an entire population without B chromosomes. (AU)

• Processo FAPESP: 18/03365-3 - INTEGRAÇÃO DE ANÁLISES CROMOSSÔMICAS E GENÔMICAS PARA O ESTUDO EVOLUTIVO E FUNCIONAL DE DNAS SATÉLITES EM Astyanax (TELEOSTEI, CHARACIFORMES)
• Beneficiário: Fábio Porto-Foresti
• Modalidade de apoio: Auxílio à Pesquisa - Regular