Extracellular Vesicle-Based Coatings Enhance Bioactivity of Titanium Implants-SurfEV

Autor(es)

Pansani, Taisa Nogueira ^[1] ; Phan, Thanh Huyen ^[2] ; Lei, Qingyu ^[2] ; Kondyurin, Alexey ^[3] ; Kalionis, Bill ^{[4, 5]} ; Chrzanowski, Wojciech ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, UNESP, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Sydney, Fac Med & Hlth, Sydney Sch Pharm, Sydney Nano Inst, Camperdown, NSW 2006 - Australia

^[3] Univ Sydney, Sydney Sch Phys, Camperdown, NSW 2006 - Australia

^[4] Royal Womens Hosp, Dept Maternal Fetal Med Pregnancy, Parkville, Vic 3052 - Australia

^[5] Univ Melbourne, Dept Obstet & Gynecol, Parkville, Vic 3052 - Australia

• Tipo de documento: Artigo Científico
• Fonte: NANOMATERIALS; v. 11, n. 6 JUN 2021.
• Citações Web of Science: 0

Resumo

Extracellular vesicles (EVs) are nanoparticles released by cells that contain a multitude of biomolecules, which act synergistically to signal multiple cell types. EVs are ideal candidates for promoting tissue growth and regeneration. The tissue regenerative potential of EVs raises the tantalizing possibility that immobilizing EVs on implant surfaces could potentially generate highly bioactive and cell-instructive surfaces that would enhance implant integration into the body. Such surfaces could address a critical limitation of current implants, which do not promote bone tissue formation or bond bone. Here, we developed bioactive titanium surface coatings (SurfEV) using two types of EVs: secreted by decidual mesenchymal stem cells (DEVs) and isolated from fermented papaya fluid (PEVs). For each EV type, we determined the size, morphology, and molecular composition. High concentrations of DEVs enhanced cell proliferation, wound closure, and migration distance of osteoblasts. In contrast, the cell proliferation and wound closure decreased with increasing concentration of PEVs. DEVs enhanced Ca/P deposition on the titanium surface, which suggests improvement in bone bonding ability of the implant (i.e., osteointegration). EVs also increased production of Ca and P by osteoblasts and promoted the deposition of mineral phase, which suggests EVs play key roles in cell mineralization. We also found that DEVs stimulated the secretion of secondary EVs observed by the presence of protruding structures on the cell membrane. We concluded that, by functionalizing implant surfaces with specialized EVs, we will be able to enhance implant osteointegration by improving hydroxyapatite formation directly at the surface and potentially circumvent aseptic loosening of implants. (AU)

• Processo FAPESP: 18/05258-0 - Desenvolvimento, caracterização e efeitos biológicos de superfície de titânio recoberta por fator de crescimento
• Beneficiário: Taisa Nogueira Pansani
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado