Metabolomic fingerprinting of Brazilian marine sponges: a case study of Plakinidae species from Fernando de Noronha Archipelago

Autor(es):Mostrar menos -

Fagundes, Thayssa da Silva F. ^[1] ; da Silva, Larissa Ramos G. ^{[2, 1]} ; Brito, Mateus de Freitas ^[1] ; Schmitz, Leticia S. S. ^[3] ; Rigato, Dhiego B. ^[3] ; Jimenez, Paula Christine ^[3] ; Soares, Angelica Ribeiro ^[4] ; Costa-Lotufo, V, Leticia ; Muricy, Guilherme ^[5] ; Vasconcelos, Thatyana Rocha A. ^[1] ; Cass, Quezia Bezerra ^[2] ; Valverde, Alessandra Leda ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Fluminense, Inst Quim, Lab Prod Nat LAPROMAR, BR-24020005 Niteroi, RJ - Brazil

^[2] Univ Fed Sao Carlos, Dept Quim, SEPARARE Nucleo Pesquisa Cromatog, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Mar, Lab Bioprospeccao Organismos Marinhos, BR-11070100 Santos, SP - Brazil

^[4] Univ Fed Rio de Janeiro, Inst Biodiversidade & Sustentabilidade NUPEM, Grp Prod Nat Organismos Aquat GPNOA, BR-27965045 Macae, RJ - Brazil

^[5] Univ Fed Rio de Janeiro, Museu Nacl, Lab Porifera, BR-20940040 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 413, n. 16, SI, p. 4301-4310, JUL 2021.
• Citações Web of Science: 0

Resumo

Marine sponges from the Plakinidae family are well known for hosting cytotoxic secondary metabolites and the Brazilian Atlantic coast and its oceanic islands have been considered as a hotspot for the discovery of new Plakinidae species. Herein, we report the chemical profile among cytotoxic extracts obtained from four species of Plakinidae, collected in Fernando de Noronha Archipelago (PE, Northeastern Brazil). Crude organic extracts of Plakinastrella microspiculifera, Plakortis angulospiculatus, Plakortis insularis, and Plakortis petrupaulensis showed strong antiproliferative effects against two different cancer cell lines (HCT-116: 86.7-100%; MCF-7: 74.9-89.5%) at 50 mu g/mL, by the MTT assay. However, at a lower concentration (5 mu g/mL), high variability in inhibition of cell growth was observed (HCT-116: 17.3-68.7%; MCF-7: 0.00-55.5%), even within two samples of Plakortis insularis which were collected in the west and east sides of the Archipelago. To discriminate the chemical profile, the samples were investigated by UHPLC-HRMS under positive ionization mode. The produced data was uploaded to the Global Natural Products Social Molecular Networking and organized based on spectral similarities for purposes of comparison and annotation. Compounds such as dipeptides, nucleosides and derivatives, polyketides, and thiazine alkaloids were annotated and metabolomic differences were perceived among the species. To the best of our knowledge, this is the first assessment for cytotoxic activity and chemical profiling for Plakinastrella microspiculifera, Plakortis insularis and Plakortis petrupaulensis, revealing other biotechnologically relevant members of the Plakinidae family. (AU)

• Processo FAPESP: 14/50244-6 - UHPLC acoplado a espectrômetro de massa do tipo híbrido com analisador quadrupolar e time of flight
• Beneficiário: Quezia Bezerra Cass
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 15/17177-6 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático