On the Use of a TiO2 Sol-Gel for Horseradish Peroxidase Enzyme Immobilization for Development of an Amperometric Biosensor

Autor(es)

da Rocha, Carolina G. ^[1] ; Uliana, V, Carolina ; Yamanaka, Hideko ^[2]

Afiliação do(s) autor(es)

^[1] Univ Esiadual Paulisia Unesp, Dept Quim Analit Fis Quim & Inorgan, Inst Quim, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

^[2] Uliana, Carolina, V, Univ Esiadual Paulisia Unesp, Dept Quim Analit Fis Quim & Inorgan, Inst Quim, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 32, n. 6, p. 1213-1221, JUN 2021.
• Citações Web of Science: 0

Resumo

The sol-gel technique has drawn considerable attention in the area of biomolecules immobilization by virtue of its intrinsic properties including chemical inertness, thermal stability, and good biocompatibility. The present work reports the development of a bio-sensing methodology which involves the immobilization of horseradish peroxidase enzyme (HRP) on pencil graphite electrode (PGE) using TiO2 sol-gel and its application for 5-aminosalicylic acid (5-ASA) determination in pharmaceutical formulations. TiO2 sol-gel film was deposited on the PGE surface by dip coating, and the HRP enzyme was immobilized on the film by adsorption and cross-linked binding using glutaraldehyde. The analytical performance of the methodology was investigated through amperometric measurements at -0.08 V in the presence of 5-ASA (electron mediator) and hydrogen peroxide (substrate). The biosensor was successfully applied for 5-ASA determination, where limits of detection and quantification of 3.3 and 10.0 µmol L -1, respectively, were obtained. The application of the methodology for 5-ASA determination in pharmaceutical formulations led to satisfactory recovery rates ranging from 80 to 98%. The biosensor developed in this work is simple, inexpensive and has the potential to be applied for the determination of other pharmaceutical compounds, which also act as electron mediators in the catalytic cycle of HRP. (AU)

• Processo FAPESP: 08/08990-1 - Estudos da lesão ao DNA por corantes têxteis e da capacidade protetora de flavonóides empregando biossensor eletroquímico
• Beneficiário: Carolina Venturini Uliana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático