| Texto completo | |
| Autor(es): |
Canon-Beltran, Karina
[1, 2]
;
Cajas, Yulia N.
[1]
;
Perez-Cerezales, Serafin
[1]
;
Leal, V, Claudia L.
;
Agirregoitia, Ekaitz
[3]
;
Gutierrez-Adan, Alfonso
[4]
;
Gonzalez, Encina M.
[5]
;
Rizos, Dimitrios
[4]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Natl Inst Agr & Food Res & Technol INIA, Dept Anim Reprod, Madrid 28040 - Spain
[2] Univ Tecn Particular Loja, Dept Ciencias Biol, Loja 110107 - Ecuador
[3] Univ Basque Country, Fac Med & Nursing, Dept Physiol, Euskal Herriko Unibertsitatea, UPV EHU, Bizkaia 48940 - Spain
[4] Leal, Claudia L., V, Natl Inst Agr & Food Res & Technol INIA, Dept Anim Reprod, Madrid 28040 - Spain
[5] Complutense Univ Madrid UCM, Vet Fac, Dept Anat & Embryol, Madrid 28040 - Spain
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC REPORTS; v. 11, n. 1 JUN 3 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
In vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF+5% FCS (Control) or supplemented with 5, 10 or 25 mu M nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (C-DMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJ(EGA)) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 +/- 1.0%) and Nob10 (44.4 +/- 1.3%) for MNEGA phase and in Nob10 (61.0 +/- 0.8%) for MJ(EGA) phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPAR alpha transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway. (AU) | |
| Processo FAPESP: | 17/20339-3 - Influência de vesículas extracelulares no metabolismo lipídico e no desenvolvimento e qualidade de embriões bovinos produzidos in vitro |
| Beneficiário: | Cláudia Lima Verde Leal |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |