| Texto completo | |
| Autor(es): |
Basso, Marcos Fernando
[1, 2]
;
Duarte, Karoline Estefani
[1, 3]
;
Santiago, Thais Ribeiro
[1, 4]
;
de Souza, Wagner Rodrigo
[3]
;
Garcia, Bruno de Oliveira
[1]
;
Brito da Cunha, Barbara Dias
[1]
;
Kobayashi, Adilson Kenji
[1]
;
Correa Molinari, Hugo Bruno
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Brazilian Agr Res Corp EMBRAPA, Natl Ctr Agroenergy Res CNPAE, BR-70770901 Brasilia, DF - Brazil
[2] Mato Grosso Cotton Inst IMAmt, BIOMOL BIOTEC Lab, BR-78740970 Rondonopolis, MT - Brazil
[3] Fed Univ ABC UFABC, Ctr Nat & Human Sci, BR-09606045 Sao Bernardo Do Campo, SP - Brazil
[4] Fed Univ Brasilia Brasilia UNB, Dept Phytopathol, BR-70910900 Brasilia, DF - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLANT BIOTECHNOLOGY; v. 38, n. 2, p. 227-238, JUN 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
The CRISPR/Cas9 system has been used for genome editing in several organisms, including higher plants. This system induces site-specific mutations in the genome based on the nucleotide sequence of engineered guide RNAs. The complex genomes of C4 grasses makes genome editing a challenge in key grass crops like maize (Zea mays), sorghum (Sorghum bicolor), Brachiaria spp., switchgrass (Panicum virgatum), and sugarcane (Saccharum spp.). Setaria viridis is a diploid C4 grass widely used as a model for these C4 crop plants. Here, an optimized CRISPR/Cas9 binary vector that exploits the non-homologous end joining (NHEJ) system was used to knockout a green fluorescent protein (gfp) transgene in S. viridis accession A10.1. Transformation of embryogenic callus by A. tumefaciens generated ten glufosinate-ammonium resistant transgenic events. In the T0 generation, 60% of the events were biallelic mutants in the gfp transgene with no detectable accumulation of GFP protein and without insertions or deletions in predicted off-target sites. The gfp mutations generated by CRISPR/Cas9 were stable and displayed Mendelian segregation in the T1 generation. Altogether, the system described here is a highly efficient genome editing system for S. viridis, an important model plant for functional genomics studies in C4 grasses. Also, this system is a potential tool for improvement of agronomic traits in C4 crop plants with complex genomes. (AU) | |
| Processo FAPESP: | 19/04878-7 - Biotecnologia aplicada ao melhoramento genético de gramíneas para a produção de biocombustíveis |
| Beneficiário: | Wagner Rodrigo de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores |