Impact of Fat Graft Thickness and Harvesting Technique on Adipocyte Viability in a New Porcine Experimental Model: An Immunohistochemical Analysis

Autor(es)

Pires de Arruda, Eduardo Gustavo ^[1] ; Munhoz, Alexandre Mendonca ^[2] ; Matsumoto, Walter ^[3] ; Ueda, Thiago ^[4] ; Montag, Eduardo ^[4] ; Okada, Alberto ^[4] ; Coudry, Renata de Almeida ^[3] ; de Castro, Isac ^[3] ; Gemperli, Rolf ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Sirio Libanes, Canc Inst Estado Sao Paulo, Dept Surg, Plast Surg Div, Sch Med, Sao Paulo - Brazil

^[2] Hosp Moriah, Plast Surg Dept, Sao Paulo - Brazil

^[3] Hosp Sirio Libanes, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Canc Inst Estado Sao Paulo, Dept Surg, Plast Surg Div, Sch Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Plast Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AESTHETIC SURGERY JOURNAL; v. 41, n. 6, p. NP616-NP630, JUN 2021.
• Citações Web of Science: 0

Resumo

Background: Autologous fat grafting (AFG) has been employed in surgical practice as a filling method. However, controversies remain on the specifics of this technique. So far, few relevant experimental large animal studies have objectively assessed factors related to AFG integration. Objectives: This study utilized an experimental, medium-sized animal model to compare the feasibility of AFG collected employing 2 different techniques with instruments of distinct thicknesses. Methods: Twenty minipigs (Sus scropha domesticus) were subjected to AFG harvesting via en bloc resection utilizing 3-(Group I) and 5-mm-diameter (Group II) round punch blades (PBs) and liposuction (LS) with 3- (Group III) and 5-mm-diameter cannulas (Group IV). Both samples were grafted intramuscularly (biceps femoralis). Hematoxylin and eosin staining was employed to identify intact adipocytes, fat necrosis, fibrosis, inflammation, and oil cysts. Immunohistochemical staining (perilipin-A, tumor necrosis factor alfa, and cluster of differentiation number 31) was utilized to quantify the feasibility of adipocytes, tissue necrosis, and neoangiogenesis, respectively. Results: Hematoxylin and eosin analysis showed that fat necrosis and histiocyte presence were significantly lower in the AFG harvested utilizing a PB than in LS. For perilipin-A, a statistical difference was observed between subgroups I and III (P = 0.001) and I and IV (P = 0.004). Instrument diameter had no effect on graft integration in comparisons between groups II and III (P = 0.059) and II and IV (P = 0.132). Conclusions: In this experimental study, fat collected utilizing a PB demonstrated higher adipocyte viability than fat collected with LS. The diameter of the collection instruments, whether PB or LS, had no effect on graft integration. (AU)

• Processo FAPESP: 14/19463-3 - Análise qualitativa da viabilidade tardia de enxertos autógenos de tecido adiposo em porcos minipig
• Beneficiário: Alexandre Mendonça Munhoz
• Modalidade de apoio: Auxílio à Pesquisa - Regular