Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells

Autor(es)

Salazar Alvarez, Luis Carlos ^{[1, 2, 3]} ; Vera Lizcano, Omaira ^{[2, 3, 4]} ; da Silva Barros, Dayanne Kamylla Alves ^{[2, 5]} ; Baia-da-Silva, Djane Clarys ^[2] ; Monteiro, Wuelton Marcelo ^{[1, 2]} ; Pimenta, Paulo Filemon Paolluci ^{[2, 6]} ; de Lacerda, Marcus Vinicius Guimaraes ^{[2, 5]} ; Costa, Fabio Trindade Maranhao ^[3] ; Lopes, Stefanie Costa Pinto ^{[2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estado Amazonas, Programa Posgrad Med Trop, Manaus, Amazonas - Brazil

^[2] Fundacao Med Trop Dr Heitor Vieira Dourado FMT HV, Manaus, Amazonas - Brazil

^[3] Univ Campinas UNICAMP, Lab Doencas Trop Prof Dr Luiz Jacintho da Silva, Dept Genet Evolucao Microbiol & Imunol, Campinas - Brazil

^[4] Univ Santiago Cali, Grp Invest QUIBIO, Fac Ciencias Basicas, Cali - Colombia

^[5] Inst Leonidas & Maria Deane ILMD Fiocruz Amazonia, Manaus, Amazonas - Brazil

^[6] Inst Rene Rachou IRR Fiocruz Minas, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 11, JUN 24 2021.
• Citações Web of Science: 0

Resumo

In a Plasmodium vivax infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage. Here, we evaluated the ex vivo cytoadhesive capacity of P. vivax gametocytes to bone marrow endothelial cells (HBMEC) and investigated the involvement of some receptors in the cytoadhesion process by using transfected CHO cells (CHO-ICAM1, CHO-CD36 and CHO-VCAM), wild type (CHO-K1) or deficient in heparan and chondroitin sulfate (CHO-745). Ex-vivo cytoadhesion assays were performed using a total of 44 P. vivax isolates enriched in gametocyte stages by Percoll gradient in the different cell lines. The majority of isolates (88.9%) were able to adhere to HBMEC monolayer. ICAM1 seemed to be the sole receptor significantly involved. CD-36 was the receptor with higher adhesion rate, despite no significance was noticed when compared to CHO-745. We demonstrated that gametocyte P. vivax adheres ex vivo to bone marrow endothelial cells. Moreover, P. vivax gametocytes display the ability to adhere to all CHO cells investigated, especially to CHO-ICAM1. These findings bring insights to the comprehension of the role of the bone marrow as a P. vivax reservoir and the potential impact on parasite transmission to the vector. (AU)

• Processo FAPESP: 17/18611-7 - Desenvolvimento de novas ferramentas para busca e validação de alvos moleculares para terapia contra Plasmodium vivax
• Beneficiário: Fabio Trindade Maranhão Costa
• Modalidade de apoio: Auxílio à Pesquisa - Temático