Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Autor(es)

Nazareth, Talita Correa ^[1] ; Zanutto, Conrado Planas ^{[2, 1]} ; Maass, Danielle ^[2] ; Ulson de Souza, Antonio Augusto ^[1] ; Guelli Ulson de Souza, Selene Maria de Arruda ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Santa Catarina UFSC, Dept Chem & Food Engn EQA, BR-88040900 Florianopolis, SC - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol ICT, BR-12231280 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING; v. 9, n. 4 AUG 2021.
• Citações Web of Science: 0

Resumo

This work reports surfactin production by B. subtilis ATCC 6051 in a stirred tank bioreactor, where trub was used as a carbon source. Trub is a residue from the brewing industry mainly composed of high-molar-mass proteins and reducing sugars, being a sustainable and low-cost substrate for bioprocess. Despite this, Trub has been little explored as a raw material in bioprocesses. Aiming to evaluate important factors in scale-up process, the impact of oxygen transfer coefficient (K(L)a) concerning volumetric productivity, and the effect of agitation (150-300 rpm) and aeration (0.5-1.5 vvm) on biosurfactant production by a Full Factorial design were evaluated. Only agitation presented a significant effect on surfactin concentration (SF), where SF was described by a linear regression model (R-2 = 0.99). The biosurfactant showed high stability in relation to variations in temperature and pH. In addition, the foam collecting was an efficient method in recovering and enrichment of the product. (AU)

• Processo FAPESP: 19/07659-4 - Valorização de metais presentes em resíduos sólidos por biomineração - BIOMETALVALUE
• Beneficiário: Danielle Maass
• Modalidade de apoio: Auxílio à Pesquisa - Regular