Electrospun Nanofibrous Architectures of Thrombin-Loaded Poly(ethylene oxide) for Faster in Vivo Wound Clotting

Autor(es)

Mendes, Larissa G. ^[1] ; Ferreira, V, Filipe ; Sielski, Micheli S. ^[2] ; Livi, Sebastien ^[3] ; Rocco, Silvana A. ^[4] ; Sforca, Mauricio L. ^[4] ; Burga-Sanchez, Jonny ^[5] ; Vicente, Cristina P. ^[2] ; Mei, I, Lucia H.

Afiliação do(s) autor(es)

^[1] I, Univ Campinas UNICAMP, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil

^[3] Univ Lyon, Ingn Mat Polymeres CNRS, UMR 5223, INSA Lyon, F-69621 Villeurbanne - France

^[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083100 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Piracicaba Dent Sch, Physiol Sci Dept, BR-13414903 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED BIO MATERIALS; v. 4, n. 6, p. 5240-5250, JUN 21 2021.
• Citações Web of Science: 1

Resumo

Wound healing materials to prevent blood loss are crucial during emergency medical treatment because uncontrolled bleeding can lead to patient death. Herein, bioabsorbable fibrous architectures of thrombin-loaded poly(ethylene oxide)-PEO/thrombin-are conceptualized and accomplished via electrospinning for faster wound clotting. Membranes with average fiber diameters ranging from 188 to 264 nm are achieved, where the active thrombin is entrapped within the nanofibers. The results of in vitro and in vivo wound healing activity tests revealed that when the nanofibers with thrombin-loaded capacity are in contact with the wound, the presence of water in the skin or blood catalyzes the degradation of the membranes, thus releasing thrombin. Thrombin then accelerates the wound clotting process. In contrast to other hemostatic materials, PEO/thrombin nanofibers do not require mechanical removal after application, and the viscoelastic nature of such biomaterials enables their conformation to a variety of wound topographies. Remarkably, PEO/thrombin membranes are promising functional materials and their use is a powerful strategy for hemostatic treatment, ranging from simple first aid and sealing to a wound to small surgical procedures. (AU)

• Processo FAPESP: 16/09588-9 - Funcionalização de nanocristais de celulose para o desenvolvimento de nanocompósitos biodegradáveis PBAT/CNC
• Beneficiário: Filipe Vargas Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado