Is there difference in the comparative and quantitative salivary proteome between stimulated and unstimulated saliva in head and neck cancer patients treated by radiotherapy

Autor(es)

Ventura, Talita Mendes Oliveira ^[1] ; Santos, Paulo Sergio Silva ^[2] ; Ribeiro, Nathalia Regina ^[1] ; Leite, Aline de Lima ^[1] ; Taira, Even Akemi ^[1] ; Dionizio, Aline ^[1] ; Rubira, Cassia Maria Fischer ^[2] ; Buzalaf, Marilia Afonso Rabelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-1701290 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Dept Surg Stomatol Pathol & Radiol, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-1701290 Bauru, SP - Brazil

• Tipo de documento: Carta
• Fonte: Oral Oncology; v. 118, JUL 2021.
• Citações Web of Science: 0

Resumo

Stimulation of saliva production is an alternative to improve the quality of life of patients treated by radiotherapy. However, there is no information about changes in the salivary proteome of stimulated and unstimulated saliva in these patients. Objectives: Thus, we evaluated the difference in the proteomic profile of stimulated and unstimulated saliva in patients with head and neck cancer (HNC) treated by radiotherapy. Methods: Stimulated and unstimulated saliva were collected from 9 patients with HNC before (BRT), during (DRT; 2-5 weeks) and after (ART; 3-4 months) treatment. Healthy patients paired by age and gender also had their saliva collected (C; control group). The stimulated and unstimulated salivary flow were evaluated (p < 0.05). Salivary proteins were extracted and processed for shotgun proteomic analysis. Results: Significant differences were observed between stimulated and unstimulated salivary flows for C and BRT (p greater than 0.001), but not for DRT and ART. Proteins involved with apoptosis, antibacterial and acid-resistance were decreased in stimulated saliva in comparison to unstimulated saliva DRT and ART. Isoforms of keratins were not identified in control and BRT. Conclusion: there is a marked difference in the protein profile of stimulated and unstimulated salivary flows in HNC patients treated by radiotherapy. In addition, saliva stimulation in patients with HNC decreases important proteins involved with dental protection. The unstimulated salivary flow seems to be the best alternative to search for biomarkers. Our results contribute in an unprecedented way to understand the changes in the salivary proteome of different flows in HNC patients undergoing radiotherapy treatment. (AU)

• Processo FAPESP: 18/17860-6 - Análise proteômica da película adquirida do esmalte em pacientes com câncer de cabeça e pescoço submetidos à radioterapia
• Beneficiário: Nathalia Regina Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica