Myotendinous Junction Components of Different Skeletal Muscles Present Morphological Changes in Obese Rats

Autor(es)

Grillo, Bruna Alexia Cristofoletti ^[1] ; Rocha, Lara C. ^[1] ; Martinez, Giovana Z. ^[1] ; Pimentel Neto, Jurandyr ^[1] ; Jacob, Carolina dos Santos ^[1] ; Watanabe, Ii-sei ^[2] ; Ciena, Adriano P. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci IB, Lab Morphol & Phys Act LAMAF, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci ICB 3, Dept Anat, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microscopy and Microanalysis; v. 27, n. 3, p. 598-603, JUN 2021.
• Citações Web of Science: 0

Resumo

Obesity is characterized by excess adipose tissue and chronic inflammation and promotes extensive changes that can compromise skeletal muscles' structural and functional integrity. Obesity can seriously impact the force transmission region between the muscle and the tendon, the myotendinous junction (MTJ). The present study aimed to investigate the plasticity of muscle fibers and MTJ regions in high-fat diet-induced obesity in rat tibialis anterior (TA) and soleus (SO) muscles. Wistar rats were divided into control and obese groups (induced by a high-fat diet). The samples of TA and SO muscles were prepared for histochemical and ultrastructural analysis (sarcomeres and MTJ projection). In the muscle fiber, similar adaptations were observed between the muscles of the smaller fiber (types I and IIa) in the obesity results. The MTJ region demonstrated different adaptations between the analyzed muscles. The TA-MTJ region has shorter ultrastructures, while in the SO-MTJ region, the ultrastructures were larger. We conclude that obesity induced by a high-fat diet promotes similar adaptation in the muscle fibers; however, in the MTJ region, the sarcoplasmatic projections and adjacent sarcomere demonstrate different adaptations according to distinct muscle phenotypes. (AU)

• Processo FAPESP: 18/20962-5 - Nova perspectiva sobre adaptação sarcomérica na junção miotendínea em modelo experimental de obesidade associado ao treinamento de esteira
• Beneficiário: Bruna Aléxia Cristofoletti Grillo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica