Ascorbic Acid Supplementation Improves Skeletal Muscle Growth in Pacu (Piaractus mesopotamicus) Juveniles: In Vivo and In Vitro Studies

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Zanella, Bruna Tereza Thomazini ^[1] ; Magiore, Isabele Cristina ^[1] ; Duran, Bruno Oliveira Silva ^[2] ; Pereira, Guilherme Gutierrez ^[1] ; Vicente, Igor Simoes Tiagua ^[3] ; Carvalho, Pedro Luiz Pucci Figueiredo ^[3] ; Salomao, Rondinelle Artur Simoes ^[4] ; Mareco, Edson Assuncao ^[4] ; Carvalho, Robson Francisco ^[1] ; de Paula, Tassiana Gutierrez ^[1] ; Barros, Margarida Maria ^[3] ; Dal-Pai-Silva, Maeli ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, UNESP, BR-18618689 Botucatu, SP - Brazil

^[2] Fed Univ Goias UFG, Inst Biol Sci, Dept Histol Embryol & Cell Biol, BR-74690900 Goiania, Go - Brazil

^[3] Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, Dept Breeding & Anim Nutr, BR-18618681 Botucatu, SP - Brazil

^[4] Univ Western Sao Paulo, Environm & Reg Dev Grad Program, BR-19050680 Presidente Prudente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 6 MAR 2021.
• Citações Web of Science: 0

Resumo

In fish, fasting leads to loss of muscle mass. This condition triggers oxidative stress, and therefore, antioxidants can be an alternative to muscle recovery. We investigated the effects of antioxidant ascorbic acid (AA) on the morphology, antioxidant enzyme activity, and gene expression in the skeletal muscle of pacu (Piaractus mesopotamicus) following fasting, using in vitro and in vivo strategies. Isolated muscle cells of the pacu were subjected to 72 h of nutrient restriction, followed by 24 h of incubation with nutrients or nutrients and AA (200 mu M). Fish were fasted for 15 days, followed by 6 h and 15 and 30 days of refeeding with 100, 200, and 400 mg/kg of AA supplementation. AA addition increased cell diameter and the expression of anabolic and cell proliferation genes in vitro. In vivo, 400 mg/kg of AA increased anabolic and proliferative genes expression at 6 h of refeeding, the fiber diameter and the expression of genes related to cell proliferation at 15 days, and the expression of catabolic and oxidative metabolism genes at 30 days. Catalase activity remained low in the higher supplementation group. In conclusion, AA directly affected the isolated muscle cells, and the higher AA supplementation positively influenced muscle growth after fasting. (AU)

• Processo FAPESP: 19/06708-1 - Aspectos morfológicos e moleculares no músculo esquelético (fibras e matriz extracelular), em pacus suplementados com ácido ascórbico: estudos in vivo e in vitro
• Beneficiário: Isabele Cristina Magiore
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/26346-1 - Efeitos da suplementação de ácido ascórbico durante a realimentação na musculatura de pacus (Piaractus mesopotamicus) submetidos a um período de jejum
• Beneficiário: Bruna Tereza Thomazini Zanella
• Modalidade de apoio: Bolsas no Brasil - Mestrado