The effect of combined curcumin-mediated photodynamic therapy and artificial skin onStaphylococcus aureus-infected wounds in rats

Autor(es)

Paolillo, Fernanda Rossi ^{[1, 2, 3]} ; Rodrigues, Phamilla Gracielli Sousa ^[3] ; Bagnato, Vanderlei Salvador ^[3] ; Alves, Fernanda ^[3] ; Pires, Layla ^[4] ; Corazza, Adalberto Vieira ^[5]

Afiliação do(s) autor(es)

^[1] State Univ Minas Gerais UEMG, Sch Phys Educ, R Colorado 700 Bairro Sao Francisco, BR-37902092 Passos, MG - Brazil

^[2] Fed Univ Alfenas UNIFAL, Motr Sci Inst, Rehabil Sci Program, Av Jovino Fernandes Sales 2600 Santa Clara, BR-37133840 Alfenas, MG - Brazil

^[3] Univ Sao Paulo, Opt Grp Phys Inst Sao Carlos IFSC, Av Trabalhador Saocarlense 400 Ctr, BR-13560970 Sao Carlos, SP - Brazil

^[4] Univ Hlth Network, Toronto Gen Res Inst, Latner Thorac Surg Res Labs, Toronto, ON M5G 2C4 - Canada

^[5] Univ Fed Mato Grosso do Sul, Sch Med, Cap Olinto Mancini Ave 1662 Colinos, BR-79600080 Tres Lagoas, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 36, n. 6, p. 1219-1226, AUG 2021.
• Citações Web of Science: 2

Resumo

Healing wounds represent a major public health problem, mainly when it is infected. Besides that, the antibiotics misuse and overuse favor the development of bacterial resistance. This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with artificial skin on disinfection of infected skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups (n= 6): (i) control-untreated; (ii) aPDT-treated with curcumin-mediated aPDT (blue light); (iii) artificial skin-treated with artificial skin alcohol-based; and (iv) aPDT plus artificial skin-treated with aPDT associated with artificial skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0.80 cm was performed in order to inoculate the microorganismStaphylococcus aureus(ATCC 25923). The aPDT was performed with a curcumin gel and a blue LED light (450 nm, 80 mW/cm(2)) at the dose of 60 J/cm(2)and the treatment with alcohol-based artificial skin was done with the topical application of 250 mu L. Additional animals were submitted to aPDT combined with the artificial skin. After treatments, the number of colony-forming units (CFU) and the damage area were determined. Data were analyzed by two-way repeated measures ANOVA and Tukey tests. The highest reduction of the bacterial viability was observed in the PDT plus artificial skin group (4.14 log10), followed by artificial skin (2.38 log10) and PDT (2.22 log10) groups. In addition, all treated groups showed higher relative area of wound contraction (36.21% for the PDT, 38.41% for artificial skin, and 35.02% for PDT plus artificial) in comparison with the control group. These findings provide evidence for the positive benefits of aPDT with blue light and curcumin associated with artificial skin to decontaminate and accelerate the wound contraction. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/14001-9 - Espectroscopia de fluorescência em dentes de ratas ovariectomizadas para o diagnóstico da osteoporose
• Beneficiário: Fernanda Rossi Paolillo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado