Effects of photobiomodulation on the redox state of healthy and cancer cells

Autor(es)

Goncalves de Faria, Clara Maria ^[1] ; Ciol, Heloisa ^[1] ; Bagnato, Vanderlei Salvador ^{[1, 2, 3]} ; Pratavieira, Sebastiao ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

^[2] Texas A&M Univ, Hagler Inst Adv Study, College Stn, TX 77843 - USA

^[3] Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 - USA

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL OPTICS EXPRESS; v. 12, n. 7, p. 3902-3916, JUL 1 2021.
• Citações Web of Science: 0

Resumo

Photobiomodulation therapy (PBMT) uses light to stimulate cells. The molecular basis of the effects of PBMT is being unveiled, but it is stated that the cytochrome-c oxidase enzyme in mitochondria, a photon acceptor of PBMT, contributes to an increase in ATP production and modulates the reduction and oxidation of electron carriers NADH and FAD. Since its effects are not fully understood, PBMT is not used on tumors. Thus, it is interesting to investigate if its effects correlate to mitochondrial metabolism and if so, how it could be linked to the optical redox ratio (ORR), defined as the ratio of FAD/(NADH + FAD) fluorescences. To that end, fibroblasts (HDFn cell line) and oral squamous cell carcinoma (SCC-25 cell line) were irradiated with a light source of 780 nm and a total dose of 5 J/cm(2), and imaged by optical microscopy. PBMT down-regulated the SCC-25 ORR by 10%. Furthermore, PBMT led to an increase in ROS and ATP production in carcinoma cells after 4 h, while fibroblasts only had a modest ATP increase 6 h after irradiation. Cell lines did not show distinct cell cycle profiles, as both had an increase in G2/M cells. This study indicates that PBMT decreases the redox state of oral cancer by possibly increasing glycolysis and affects normal and tumor cells through distinct pathways. To our knowledge, this is the first study that investigated the effects of PBMT on mitochondrial metabolism from the initiation of the cascade to DNA replication. This is an essential step in the investigation of the mechanism of action of PBMT in an effort to avoid misinterpretations of a variety of combined protocols. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement (AU)

• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/14182-4 - Desenvolvimento de técnica combinando fotobiomodulação e radioterapia para otimização da resposta tumoral à radiação ionizante
• Beneficiário: Clara Maria Gonçalves de Faria
• Modalidade de apoio: Bolsas no Brasil - Doutorado