Purification and characterization of two new antimicrobial molecules produced by an endophytic strain of Paenibacillus polymyxa

Autor(es)

Serrano, Nadja F. G. ^[1] ; Ferreira, Douglas ^[2] ; Rodrigues-Filho, Edson ^[3] ; Teixeira, Jose Antonio ^[4] ; Dubreuil, Jean Daniel ^[5] ; De Sousa, Cristina P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, Dept Morfol & Patol, Lab Microbiol & Biomoleculas, Programa Posgrad Biotecnol, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Oeste Bahia, Dept Quim, Recanto Passaros, Campus Luis Eduardo Magalhaes, Rua Prof Jose Seabr, BR-47808021 Barreiras, BA - Brazil

^[3] Univ Fed Sao Carlos, Lab Bioquim Micromol Microrganismos, Dept Quim, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Minho, Ctr Engn Biol, Lab Fermentacoes, Rua Univ, P-4710057 Braga - Portugal

^[5] Univ Montreal, Fac Med Vet, Dept Pathol & Microbiol, 3200 Rue Sicotte, St Hyacinthe, PQ J2S 2M2 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Anais da Academia Brasileira de Ciências; v. 93, n. 3 2021.
• Citações Web of Science: 0

Resumo

Abstract An endophytic bacterium inhibiting pathogenic bacteria was isolated and the strain was genetically identified as Paenibacillus polymyxa. Biochemical characterization of fermentation broth indicated the presence of peptidic antimicrobial molecules. Liquid-liquid partition resulted in an organic fraction (OF) and an aqueous fraction (AF). OF presented a broad spectrum of activity against a panel of pathogenic bacteria and a fungus whereas the AF was active only against Gram-negative bacteria. AF was sequentially submitted to ion-exchange, desalting and reverse phase (RP) chromatography. A molecule with an RT of 2.45 min exhibited activity against all Gram-negative pathogenic strains tested beside P. mirabilis. The primary structure of the molecule, named AMP-Pp, was determined as Gly-Glu-Hyp-Gly-Ala by N-terminal sequencing. The molecular mass and amino acid sequence were confirmed by MS/MS. With a molecular mass of 463 Da, AMP-Pp is one of the smallest active natural peptides reported, yet. RP chromatography of OF resulted in four peaks. The first three peaks corresponded to known antimicrobials. MS analysis of peak 4 revealed the presence of an ion with m/z 3,376.4 Da, whose proposed molecular formula is C182H321N29O29. The compound, named polycerradin, showed a spectrum of activity against Gram-positive bacteria, Gram-negative bacteria (beside P. mirabilis) and a fungus. (AU)

• Processo FAPESP: 16/13423-5 - Compostos produzidos por microrganismos endofíticos isolados de plantas do cerrado: bioatividade, associação com a-Ag2WO4 e aplicações biotecnológicas
• Beneficiário: Cristina Paiva de Sousa
• Modalidade de apoio: Auxílio à Pesquisa - Regular