The Protein-Independent Role of Phosphate in the Progression of Chronic Kidney Disease

Autor(es):Mostrar menos -

Duayer, Irene Faria ^[1] ; Duque, Eduardo Jorge ^[1] ; Fujihara, Clarice Kazue ^[1] ; de Oliveira, Ivone Braga ^[1] ; dos Reis, Luciene Machado ^[1] ; Machado, Flavia Gomes ^[1] ; Graciolli, Fabiana Giorgetti ^[1] ; Jorgetti, Vanda ^[1] ; Zatz, Roberto ^[1] ; Elias, Rosilene Motta ^{[1, 2]} ; Affonso Moyses, Rosa Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin HCFMUSP, Nephrol Dept, LIM 16, BR-05403000 Sao Paulo - Brazil

^[2] Univ Nove de Julho UNINOVE, Postgrad, BR-01525000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 13, n. 7 JUL 2021.
• Citações Web of Science: 0

Resumo

Several factors contribute to renal-function decline in CKD patients, and the role of phosphate content in the diet is still a matter of debate. This study aims to analyze the mechanism by which phosphate, independent of protein, is associated with the progression of CKD. Adult Munich-Wistar rats were submitted to 5/6 nephrectomy (Nx), fed with a low-protein diet, and divided into two groups. Only phosphate content (low phosphate, LoP, 0.2%; high phosphate, HiP, 0.95%) differentiated diets. After sixty days, biochemical parameters and kidney histology were analyzed. The HiP group presented worse renal function, with higher levels of PTH, FGF-23, and fractional excretion of phosphate. In the histological analysis of the kidney tissue, they also showed a higher percentage of interstitial fibrosis, expression of alpha-actin, PCNA, and renal infiltration by macrophages. The LoP group presented higher expression of beclin-1 in renal tubule cells, a marker of autophagic flux, when compared to the HiP group. Our findings highlight the action of phosphate in the induction of kidney interstitial inflammation and fibrosis, contributing to the progression of renal disease. A possible effect of phosphate on the dysregulation of the renal cell autophagy mechanism needs further investigation with clinical studies. (AU)

• Processo FAPESP: 10/05409-6 - Efeito da sobrecarga de proteína e de fósforo na progressão da doença renal: avaliação dos mecanismos fisiopatológicos
• Beneficiário: Rosa Maria Affonso Moysés
• Modalidade de apoio: Auxílio à Pesquisa - Regular