Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (Chondrilla caribensis and Aplysina fulva): Physicochemical Properties and In Vitro Biological Analysis

Autor(es):Mostrar menos -

Araujo, Tiago A. T. ^[1] ; de Souza, Amanda ^[1] ; Santana, Alan F. ^[1] ; Braga, Anna Rafaela C. ^[1] ; Custodio, Marcio R. ^[2] ; Simoes, Fabio R. ^[3] ; Araujo, Gabriela M. ^[3] ; Miranda, Antonio ^[4] ; Alves, Flavio ^[4] ; Granito, Renata N. ^[1] ; Yu, Na ^[5] ; Renno, Ana Claudia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Biosci, BR-11015220 Santos, SP - Brazil

^[2] Univ Sao Paulo, Inst Biosci, Lab Marine Invertebrates Cell Biol, BR-05508090 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Inst Marine Sci, BR-11070100 Santos, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Biophys, BR-04044020 Sao Paulo, SP - Brazil

^[5] Natl Dent Ctr Singapore, 5 Second Hosp Ave, Singapore 168938 - Singapore

• Tipo de documento: Artigo Científico
• Fonte: MEMBRANES; v. 11, n. 7 JUL 2021.
• Citações Web of Science: 0

Resumo

This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from Chondrilla caribensis and the nodular/particulate aggregates for Aplysina fulva. FTIR showed for all samples peaks similar to collagen for both species. For C. caribensis, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For A. fulva, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis; also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for C. caribensis was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all A. fulva extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from C. caribensis. (AU)

• Processo FAPESP: 14/23858-3 - Desenvolvimento de materiais de sensores baseados em filmes automontados de polímeros condutores e nanotubos de carbono para a determinação de desreguladores endócrinos em ambientes marinhos
• Beneficiário: Fabio Ruiz Simões
• Modalidade de apoio: Auxílio à Pesquisa - Regular