Marker-assisted backcrossing for disease resistance and agronomic traits in Carioca beans

Autor(es)

de Almeida, Caleo Panhoca ^[1] ; Paulino, Jean Fausto de Carvalho ^[1] ; Santos, Isabella Laporte ^[1] ; Bajay, Miklos Maximiliano ^[2] ; Goncalves, Joao Guilherme Ribeiro ^[3] ; Carvalho, Cassia Regina Limonta ^[1] ; Carbonell, Sergio Augusto Morais ^[3] ; Chiorato, Alisson Fernando ^[3] ; Benchimol-Reis, Luciana Lasry ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Pesquisa Recursos Genet Inst Agron IAC, Campinas, SP - Brazil

^[2] Univ Estado Santa Catarina, Ctr Educ Super Regiao Sul, Laguna, SC - Brazil

^[3] Ctr Graos & Fibras Inst Agron IAC, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CROP SCIENCE; v. 61, n. 4, p. 2510-2521, JUL 2021.
• Citações Web of Science: 0

Resumo

Angular leaf spot, a disease caused by the fungus Pseudocercospora griseola, can lead to yield losses of up to 70% in the common bean (Phaseolus vulgaris) crop. Due to the quantitative character of angular leaf spot, a cultivar with high resistance depends on the presence of several resistance loci. In the present study, a marker-assisted backcrossing approach was used involving an Andean donor parent (AND 277) and a Mesoamerican recurrent parent (IAC-Milenio). For marker-assisted backcrossing in the BC1F1 genotypes, 15 molecular markers previously mapped for angular leaf spot resistance loci were used, and selection was performed by genotypes grouped with the donor parent in discrimination analysis of principal components. Through molecular selection, 42% of the BC2F3 families selected for the Carioca grain ideotype showed high resistance to angular leaf spot under controlled conditions of infection. Two lines resistant under natural conditions and tolerant to Fusarium wilt (Fusarium oxysporum f. sp. phaseoli J. B. Kendr. \& W. C. Snyder) were selected for the competition trial. The lines selected, AM138 and AM167, showed superiority to the recurrent cultivar for six of the eight agronomic traits, including resistance to Race 65 of Colletotrichum lindemuthianum slow grain darkening, early maturity, and lighter grain color. Both advanced lines will be included in the value for cultivation and use trials; and as Carioca lines with resistance of Andean origin, they can be widely used as sources of resistance in breeding programs to overcome problems related to inter-gene pool crosses. (AU)

• Processo FAPESP: 14/11145-2 - Seleção assistida por marcadores moleculares pelo método de AB-QTL e por genotipagem por sequenciamento (GBS) visando à resistência à mancha angular e antracnose em feijão comum
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/01753-3 - Seleção assistida por marcadores moleculares e genotipagem por sequenciamento (GBS) visando à resistência à mancha angular em feijão comum
• Beneficiário: Caléo Panhoca de Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado