Inflammatory and neurotrophic factor plasma levels are related to epilepsy independently of etiology

Autor(es):Mostrar menos -

Alvim, Marina K. M. ^[1] ; Morita-Sherman, Marcia E. ^{[1, 2]} ; Yasuda, Clarissa L. ^[1] ; Rocha, Natalia P. ^[3] ; Vieira, Erica L. ^[3] ; Pimentel-Silva, Luciana R. ^[1] ; Henrique Nogueira, Mateus ^[1] ; Barbosa, Renata ^[1] ; Watanabe, Nancy ^[1] ; Coan, Ana Carolina ^[1] ; Lopes-Cendes, Iscia. ^[1] ; Teixeira, Antonio L. ^{[3, 2]} ; Cendes, Fernando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Campinas - Brazil

^[2] Cleveland Clin Fdn, Epilepsy Ctr, 9500 Euclid Ave, Cleveland, OH 44195 - USA

^[3] Univ Fed Minas Gerais, Sch Med, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Epilepsia; v. 62, n. 10 JUL 2021.
• Citações Web of Science: 0

Resumo

Objective Inflammation plays an essential role in epilepsy. Studies indicate that cytokines and neurotrophic factors can act in neuroexcitability and epileptogenesis. We aimed to investigate the association between plasma inflammatory and neurotrophic markers, seizure frequency, and chronic epilepsy subtypes. Methods We studied 446 patients with epilepsy and 166 healthy controls. We classified patients according to etiology and seizure frequency. We measured plasma levels of interleukin-1 (IL-1), IL-2, IL-4, IL-6, IL-10, IL-17, interferon-gamma (IFN gamma), tumor necrosis factor alpha (TNF alpha), soluble TNF receptor 1 (sTNFr1), sTNFr2, brain-derived neurotrophic factor (BDNF), neurotrophic factor 3 (NT3), NT4/5, ciliary neurotrophic factor (CNTF), nerve growth factor (NGF), and glial cell line-derived neurotrophic factor (GDNF) by enzyme-linked immunosorbent assay or cytometric bead array. Results The plasma levels of BDNF, NT3, NGF, and sTNFr2 were higher, whereas IL-2, IL-4, IL-6, IL-10, IL-17, IFN gamma, TNF alpha, CNTF, and sTNFr1 were lower in patients than controls. IL1, GDNF, and NT4/5 were similar between groups. These markers did not correlate with age, sex, and epilepsy duration. The molecule sTNFr2 was the best marker to discriminate patients from controls (area under the curve = .857), also differing between patients with frequent and infrequent seizures. Significance This large cohort confirmed that patients with epilepsy have abnormal levels of plasma inflammatory and neurotrophic markers independent of the underlying etiology. Plasma level of sTNFr2 was related to seizure frequency and discriminated people with or without epilepsy with good accuracy, making it a potential biomarker for epilepsy and seizure burden. (AU)

• Processo FAPESP: 13/07559-3 - Instituto Brasileiro de Neurociência e Neurotecnologia - BRAINN
• Beneficiário: Fernando Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/17066-0 - Avaliação da relação entre marcadores inflamatórios e padrão de atrofia hipocampal e extra-hipocampal em pacientes com epilepsia de lobo temporal
• Beneficiário: Marina Koutsodontis Machado Alvim
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/53442-5 - Análise da relação entre expressão de vias inflamatórias sistêmicas, epileptogênese e atrofia cerebral nas epilepsias parciais
• Beneficiário: Fernando Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Regular