Galectin-3 immunolabelling correlates with BCL2 expression in canine cutaneous mast cell tumours

Autor(es)

Vargas, Thiago Henrique M. ^[1] ; Barra, Camila N. ^{[2, 1]} ; Pulz, Lidia H. ^{[2, 1]} ; Huete, Greice C. ^[1] ; Cadrobbi, Karine G. ^[1] ; Nishiya, Adriana Tomoko ^[3] ; Kleeb, Silvia Regina ^[3] ; Xavier, Jose Guilherme ^[4] ; Catao-Dias, Jose Luiz ^[2] ; Strefezzi, Ricardo F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Lab Oncol Comparada & Translac LOCT, Av Duque de Caxias Norte 225, BR-13635900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Sao Paulo, SP - Brazil

^[3] Univ Anhembi Morumbi, Sao Paulo, SP - Brazil

^[4] Univ Paulista, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA VETERINARIA HUNGARICA; v. 69, n. 2, p. 169-174, JUN 2021.
• Citações Web of Science: 0

Resumo

Mast cell tumour (MCT) is the most frequent skin neoplasm in dogs. These tumours are characterised by variable behaviour and clinical presentation that make prognosis an important and challenging task in the veterinary practice. Galectin-3 (Gal-3) is known to influence several biological processes that are important in the cancer context and has been described as a prognostic marker for several human cancers. The aim of the present work was to characterise Gal-3 immunolabelling in canine cutaneous MCTs and to investigate its value as a prognostic marker for the disease. Thirty-four random cases of canine cutaneous MCT that were surgically treated with wide margins were included in this study. Gal-3 expression was evaluated using immunohistochemistry and the results were compared with the expression of apoptosis-related proteins, Ki67 index, histopathological grades, mortality due to the disease and post-surgical survival. The majority of the MCTs (65.8%) were positive for Gal-3. Gal-3 immunolabelling was variable among the samples (2.7%-86.8% of the neoplastic cells). The protein was located in the cytoplasm or in the cytoplasm and the nucleus. Gal-3 positivity was correlated with BCL2 expression (P < 0.001; r = 0.604), but not with Ki67 and BAX. No significant differences were detected between histological grades or in the survival analysis. Gal-3 expression correlates with BCL2 expression in MCTs. Although an efficient marker for several human neoplasms, the results presented herein suggest that Gal-3 immunolabelling is not an independent prognostic indicator for this disease. (AU)

• Processo FAPESP: 13/13252-8 - Caracterização de genes relacionados à apoptose em mastocitomas cutâneos caninos e seu valor como indicador prognóstico
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11959-2 - Caracterização imuno-histoquímica da galectina-3 como ferramenta prognóstica em melanomas orais caninos
• Beneficiário: Thiago Henrique Moroni Vargas
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/03862-1 - Caracterização de fibroblastos associados ao câncer e sua influência sobre neoplasias caninas
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular