How to characterize a strain? Clonal heterogeneity in industrial Saccharomyces influences both phenotypes and heterogeneity in phenotypes

Autor(es)

Racz, Hanna Viktoria ^{[1, 2]} ; Mukhtar, Fezan ^[1] ; Imre, Alexandra ^{[1, 3]} ; Radai, Zoltan ^[4] ; Gombert, Andreas Karoly ^[5] ; Ratonyi, Tamas ^[6] ; Nagy, Janos ^[6] ; Pocsi, Istvan ^[1] ; Pfliegler, Walter P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Debrecen, Dept Mol Biotechnol & Microbiol, Egyet Ter 1, H-4032 Debrecen - Hungary

^[2] Univ Debrecen, Doctoral Sch Nutr & Food Sci, Debrecen - Hungary

^[3] Univ Debrecen, Kalman Laki Doctoral Sch Biomed & Clin Sci, Debrecen - Hungary

^[4] Inst Ecol & Bot, MTA OK Lendulet Seed Ecol Res Grp, Ctr Ecol Res, Vacratot - Hungary

^[5] Univ Estadual Campinas, Sch Food Engn, Campinas, SP - Brazil

^[6] Univ Debrecen, Inst Land Use Technol & Reg Dev, Debrecen - Hungary

• Tipo de documento: Artigo Científico
• Fonte: YEAST; v. 38, n. 8, p. 453-470, AUG 2021.
• Citações Web of Science: 0

Resumo

Populations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is nontrivial to assess methodologically. The necessary practice of isolating single-cell colonies and thus subclone lineages for establishing, transferring, and using a strain results in single-cell bottlenecks with a generally neglected effect on the characteristics of the strain itself. Here, we present evidence that various subclone lineages for industrial yeasts sequenced for recent genomic studies show considerable differences, ranging from loss of heterozygosity to aneuploidies. Subsequently, we assessed whether phenotypic heterogeneity is also observable in industrial yeast, by individually testing subclone lineages obtained from products. Phenotyping of industrial yeast samples and their newly isolated subclones showed that single-cell bottlenecks during isolation can indeed considerably influence the observable phenotype. Next, we decoupled fitness distributions on the level of individual cells from clonal interference by plating single-cell colonies and quantifying colony area distributions. We describe and apply an approach using statistical modeling to compare the heterogeneity in phenotypes across samples and subclone lineages. One strain was further used to show how individual subclonal lineages are remarkably different not just in phenotype but also in the level of heterogeneity in phenotype. With these observations, we call attention to the fact that choosing an initial clonal lineage from an industrial yeast strain may vastly influence downstream performances and observations on karyotype, on phenotype, and also on heterogeneity. (AU)

• Processo FAPESP: 18/04962-5 - Evolução microbiana na indústria de etanol combustível no Brasil
• Beneficiário: Andreas Karoly Gombert
• Modalidade de apoio: Bolsas no Exterior - Pesquisa