ATP regulates the activity of an alternative oxidase in Trypanosoma brucei

Autor(es)

Luevano-Martinez, Luis Alberto ^[1] ; Girard, Richard M. B. M. ^[1] ; Alencar, Mayke Bezerra ^[1] ; Silber, Ariel Mariano ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Lab Biochem Tryps LaBTryps, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS Letters; v. 594, n. 13, p. 2150-2158, JUL 2020.
• Citações Web of Science: 3

Resumo

The reduced mitochondrial respiratory chain from the bloodstream forms of Trypanosoma brucei is composed of only a membrane-bound glycerol-3-phosphate dehydrogenase and an alternative oxidase. Since these enzymes are not proton pumps, their functions are restricted to the maintenance of the redox balance in the glycosome by means of the dihydroxyacetone phosphate/glycerol-3-phosphate shuttle. Additionally, an F1Fo-ATP synthase functions as an ATP-hydrolysing enzyme to establish the proton motive force necessary to maintain the basic functions of mitochondria. In this report, we studied the interplay between the alternative oxidase and ATP synthase, and we found that, in addition to its role as a proton pump, ATP synthase contributes to maintain safe levels of ATP to prevent the inhibition of the alternative oxidase by ATP. (AU)

• Processo FAPESP: 16/06034-2 - O papel biológico de aminoácidos e seus metabólitos derivados em Trypanosoma cruzi
• Beneficiário: Ariel Mariano Silber
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/12999-0 - Evolução da via de síntese da cardiolipina: implicações bioquímicas na evolução do domínio Eukarya
• Beneficiário: Luis Alberto Luevano Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores