An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth

Autor(es)

Duran, Bruno Oliveira Silva ^{[1, 2]} ; Garcia de la Serrana, Daniel ^[3] ; Zanella, Bruna Tereza Thomazini ^[1] ; Perez, Erika Stefani ^[1] ; Mareco, Edson Assuncao ^[4] ; Santos, Vander Bruno ^[5] ; Carvalho, Robson Francisco ^[1] ; Dal-Pai-Silva, Maeli ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP - Brazil

^[2] Fed Univ Goias UFG, Inst Biol Sci, Dept Histol Embryol & Cell Biol, Goiania, Go - Brazil

^[3] Univ Barcelona, Fac Biol, Dept Cell Biol Physiol & Immunol, Barcelona - Spain

^[4] Univ Western Sao Paulo UNOESTE, Sao Paulo - Brazil

^[5] Fisheries Inst IP APTA, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 7 JUL 22 2021.
• Citações Web of Science: 0

Resumo

Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350-320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene repertoire. Duplicated genes can be retained by different molecular mechanisms such as subfunctionalization, neofunctionalization or redundancy, each one with different functional implications. While the great majority of ohnolog genes have been identified in the teleost genomes, the effect of gene duplication in the fish physiology is still not well characterized. In the present study we studied the effect of WGD on the transcription of the duplicated components controlling muscle growth. We compared the expression of lineage-specific ohnologs related to myogenesis and protein balance in the fast-skeletal muscle of pacus (Piaractus mesopotamicus-Ostariophysi) and Nile tilapias (Oreochromis niloticus-Acanthopterygii) fasted for 4 days and refed for 3 days. We studied the expression of 20 ohnologs and found that in the great majority of cases, duplicated genes had similar expression profiles in response to fasting and refeeding, indicating that their functions during growth have been conserved during the period after the WGD. Our results suggest that redundancy might play a more important role in the retention of ohnologs of regulatory pathways than initially thought. Also, comparison to non-duplicated orthologs showed that it might not be uncommon for the duplicated genes to gain or loss new regulatory elements simultaneously. Overall, several of duplicated ohnologs have similar transcription profiles in response to pro-growth signals suggesting that evolution tends to conserve ohnolog regulation during muscle development and that in the majority of ohnologs related to muscle growth their functions might be very similar. (AU)

• Processo FAPESP: 18/24575-6 - Análises integrativas e comparativas do fosfoproteoma de culturas celulares de mioblastos do pacu (Piaractus mesopotamicus) e gilthead seabream (Sparus aurata) submetidas a tratamentos com fatores de crescimento.
• Beneficiário: Bruno Oliveira da Silva Duran
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/26428-0 - Compreensão das interações entre o fosfoproteoma, transcriptoma e microRNAoma em resposta a sinais pró-crescimento em miotubos de pacu (Piaractus mesopotamicus) e gilthead sea bream (Sparus aurata).
• Beneficiário: Maeli Dal Pai
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03234-8 - Cultura de mioblastos do pacu (Piaractus mesopotamicus) como um modelo para entender a regulação do crescimento muscular na superordem Ostariophysi
• Beneficiário: Bruno Oliveira da Silva Duran
• Modalidade de apoio: Bolsas no Brasil - Doutorado