Field microenvironments regulate crop diel transcript and metabolite rhythms

Autor(es)

Dantas, Luiza Lane Barros ^{[1, 2]} ; Dourado, Maira Marins ^[1] ; de Lima, Natalia Oliveira ^[1] ; Cavacana, Natale ^[1] ; Nishiyama, Jr., Milton Yutaka ^[3] ; Souza, Glaucia Mendes ^[1] ; Carneiro, Monalisa Sampaio ^[4] ; Caldana, Camila ^[5] ; Hotta, Carlos Takeshi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil

^[2] John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk - England

^[3] Inst Butantan, Lab Especial Toxicol Aplicada, BR-05503900 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Carlos, Ctr Ciencias Agr, Dept Biotecnol & Prod Vegetal & Anim, BR-13600970 Sao Carlos, SP - Brazil

^[5] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam - Germany

• Tipo de documento: Artigo Científico
• Fonte: NEW PHYTOLOGIST; v. 232, n. 4 AUG 2021.
• Citações Web of Science: 0

Resumo

Most research in plant chronobiology has been done in laboratory conditions. However, laboratories usually fail to mimic natural conditions and their slight fluctuations, highlighting or obfuscating rhythmicity. High-density crops, such as sugarcane (Saccharum hybrid), generate field microenvironments with specific light and temperature regimes resulting from mutual shading. We measured the metabolic and transcriptional rhythms in the leaves of 4-month-old (4 mo) and 9 mo field-grown sugarcane. Most of the assayed rhythms in 9 mo sugarcane peaked >1 h later than in 4 mo sugarcane, including rhythms of the circadian clock gene, LATE ELONGATED HYPOCOTYL (LHY). We hypothesized that older sugarcane perceives dawn later than younger sugarcane as a consequence of self-shading. As a test, we measured LHY rhythms in plants on the east and the west sides of a field. We also tested if a wooden wall built between lines of sugarcane plants changed their rhythms. The LHY peak was delayed in the plants in the west of the field or beyond the wall; both shaded at dawn. We conclude that plants in the same field may have different phases resulting from field microenvironments, impacting important agronomical traits, such as flowering time, stalk weight and number. (AU)

• Processo FAPESP: 11/00818-8 - Desenvolvimento de modelos biológicos alternativos para o estudo de redes de regulação em cana-de-açúcar
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores
• Processo FAPESP: 16/06740-4 - Caracterização do relógio biológico em tecidos fotossintéticos de plantas C4
• Beneficiário: Natalia Oliveira de Lima
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/08897-4 - Caracterização do relógio biológico e seu impacto no metabolismo da cana-de-açúcar
• Beneficiário: Luíza Lane de Barros Dantas
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06260-0 - Relógios biológicos específicos em órgãos e tecidos de gramíneas C4
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 19/08534-0 - Caracterização do relógio biológico de genótipos de cana-de-açúcar contrastantes para sacarose e fibra
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 17/50326-0 - Diurnal regulation of carbon metabolism in plants
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Regular