Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis

Autor(es)

Giglioti, Rodrigo ^[1] ; Okino, Cintia Hiromi ^[2] ; Azevedo, Bianca Taina ^[1] ; Rodrigues Wedy, Bruna Costa ^[1] ; Gutmanis, Gunta ^[1] ; Verissimo, Cecilia Jose ^[1] ; Katiki, Luciana Morita ^[1] ; Vercesi Filho, Anibal Eugenio ^[1] ; de Oliveira, Henrique Nunes ^[3] ; de Sena Oliveira, Marcia Cristina ^[2]

Afiliação do(s) autor(es)

^[1] Inst Zootecnia, Rua Heitor Penteado 56, BR-13380011 Nova Odessa, SP - Brazil

^[2] Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil

^[3] Univ Estadual Julio de Mesquita Filho, Unesp Jaboticabal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TICKS AND TICK-BORNE DISEASES; v. 12, n. 5 SEP 2021.
• Citações Web of Science: 0

Resumo

Bovine babesiosis is economically the most important arthropod-borne disease of cattle worldwide. The most significant damage caused by bovine babesiosis is attributed to Babesia bovis due to its higher pathogenicity. This study aimed to develop a real-time PCR method followed by HRM (high-resolution melting) analysis for the simultaneous detection of B. bovis and B. bigemina, enabling a semi-quantitative analysis of Babesia levels using a single-tube reaction. The HRM was compared with real-time PCR using species-specific hydrolysis probes. The HRM analysis allowed to differentiate both Babesia species and was sensitive in the detection and differentiation of 10% for each Babesia species in the sample. Our results suggest the use of this method to estimate the prevalence of infections by B. bovis or B. bigemina as an alternative to the methods of absolute quantification by real-time PCR since it neither requires precise estimates of the number of DNA loads nor the construction of calibration curves. The simultaneous detection of the two Babesia species can be used to characterise the infection levels in cattle populations from different geographical regions, allowing a better control of these diseases. (AU)

• Processo FAPESP: 19/22675-6 - Análise transcriptômica da resistência e/ou suscetibilidade de bovinos de corte de diferentes grupos genéticos à infecção por Babesia bovis
• Beneficiário: Rodrigo Giglioti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/07216-7 - Estudo genômico e caracterização imunológica de bovinos resistentes à babesiose bovina e análise da diversidade genética de Babesia bovis e Babesia bigemina
• Beneficiário: Henrique Nunes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/19452-2 - Equipamento multiusuário concedido no Processo FAPESP 2017/50.339-5: nanoespectrofotômetro BioDrop
• Beneficiário: Renata Helena Branco Arnandes
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários