Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4 `-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma

Autor(es):Mostrar menos -

Seba, Viviane ^{[1, 2]} ; de Lima, Gabriel Goetten ^{[2, 3]} ; Pereira, Bruno L. ^[3] ; Silva, Gabriel ^[4] ; Reinhardt, Luiza Steffens ^{[5, 6]} ; Arantes, Pablo Ricardo ^[7] ; Chee, Bor Shin ^[2] ; dos Santos, Mariana Bastos ^[8] ; Franca, Suzelei C. ^[1] ; Regasini, Luis Octavio ^[8] ; Fachin, Ana Lucia ^{[1, 9]} ; Cao, Zhi ^[2] ; Nugent, Michael J. D. ^[2] ; Marins, Mozart ^{[1, 9, 10]}

Afiliação do(s) autor(es)

^[1] Univ Ribeirao Preto, Biotechnol Unit, BR-14096900 Ribeirao Preto, SP - Brazil

^[2] Athlone Inst Technol, Mat Res Inst, Athlone, Westmeath - Ireland

^[3] Univ Fed Parana, Post Grad Program Engn & Mat Sci PIPE, BR-81530000 Curitiba, PR - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040900 Ribeirao Preto, SP - Brazil

^[5] Fed Univ Hlth Sci Porto Alegre, Lab Genet Toxicol, BR-90050170 Porto Alegre, RS - Brazil

^[6] Univ Newcastle, Coll Hlth Med & Wellbeing, Hunter Med, Sch Biomed Sci & Pharm, Newcastle, NSW 2308 - Australia

^[7] Univ Calif Riverside, Dept Bioengn, Riverside, CA 92507 - USA

^[8] Sao Paulo State Univ Unesp, Inst Biosci Humanities & Exact Sci, Dept Chem & Environm Sci, Lab Green & Med Chem, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[9] Univ Ribeirao Preto, Med Sch, BR-14096900 Ribeirao Preto, SP - Brazil

^[10] Univ Ribeirao Preto, Pharmaceut Sci Sch, BR-14096900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: POLYMERS; v. 13, n. 16 AUG 2021.
• Citações Web of Science: 0

Resumo

Chalcones (1,3-diaryl-2-propen-1-ones) are naturally occurring polyphenols with known anticancer activity against a variety of tumor cell lines, including osteosarcoma (OS). In this paper, we present the preparation and characterization of spheres (similar to 2 mm) from polyvinyl alcohol (PVA) containing a combination of 4 `-Amino-1-Naphthyl-Chalcone (D14) and doxorubicin, to act as a new polymeric dual-drug anticancer delivery. D14 is a potent inhibitor of osteosarcoma progression and, when combined with doxorubicin, presents a synergetic effect; hence, physically crosslinked PVA spheres loaded with D14 and doxorubicin were prepared using liquid nitrogen and six freeze-thawing cycles. Physical-chemical characterization using a scanning electron microscope (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) presented that the drugs were incorporated into the spheres via weak interactions between the drugs and the polymeric chains, resulting in overall good drug stability. The cytotoxicity activity of the PVA spheres co-encapsulating both drugs was tested against the U2OS human osteosarcoma cell line by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and compared to the spheres carrying either D14 or doxorubicin alone. The co-delivery showed a cytotoxic effect 2.6-fold greater than doxorubicin alone, revealing a significant synergistic effect with a coefficient of drug interaction (CDI) of 0.49. The obtained results suggest this developed PVA sphere as a potential dual-drug delivery system that could be used for the prominent synergistic anticancer activity of co-delivering D14 and doxorubicin, providing a new potential strategy for improved osteosarcoma treatment. (AU)

• Processo FAPESP: 17/03237-2 - Bioprospecção de polifenóis com atividade anticancerígena e investigação dos mecanismos moleculares envolvendo o fator de transcrição p53
• Beneficiário: Mozart de Azevedo Marins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/03074-1 - Explorando a atividade do fator de transcrição Sp1 associada a transição epitélio mesenquimal como alvo terapêutico do osteossarcoma
• Beneficiário: Mozart de Azevedo Marins
• Modalidade de apoio: Auxílio à Pesquisa - Regular