Angiotensin involvement in kidney injury induced by rheumatoid arthritis in rat

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Carlos, Carla Patricia ^[1] ; de Carvalho, Enzo Prandi ^[1] ; Angeli Junior, Euradir Vitorio ^[1] ; Garcia Filho, Glayber Falcao ^[1] ; Dona, Joao Pedro Lot ^[1] ; Batanero, Rodrigo Piloto de Oliveira ^[1] ; Guena, Rafael de Oliveira ^[1] ; Agren, Camila ^[1] ; Baptista, Maria Alice Sperto Ferreira ^[2] ; Bizotto, Thais Santana Gastardelo ^[3] ; Cury, Patricia Maluf ^[1] ; Chies, Agnaldo Bruno ^[4]

Afiliação do(s) autor(es)

^[1] FACERES Sch Med, Lab Expt Res, Sao Jose Do Rio Preto, SP - Brazil

^[2] FUNFARME FAMERP Sch Med, Dept Nephrol & Pathol, Sao Jose Do Rio Preto, SP - Brazil

^[3] FUNFARME FAMERP Sch Med, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

^[4] FAMEMA, Lab Pharmacol, Marilia Med Sch, Sao Paulo, Marilia - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical and Experimental Pharmacology and Physiology; v. 48, n. 9, p. 1271-1279, SEP 2021.
• Citações Web of Science: 0

Resumo

Renal injury induced by rheumatoid arthritis is not clear and may be related to the angiotensin II. We aim to investigate the adjuvant-induced arthritis (AIA) injury in rat kidney, focusing the angiotensin II/AT(1) pathway. Male Wistar rats were allocated in to three groups: Control, AIA and AIA plus losartan. The AIA was induced by injection of 100 mu L of an emulsion of dissected Mycobacterium tuberculosis (50 mg/mL) on the paw. Treatment with losartan was initiated on the first day of immunization (daily subcutaneous injection, 1 mg/kg). After 60 days post immunization, we evaluated kidney function by plasma creatinine, urea and uric acid levels and creatinine depuration; kidney injury by apoptosis analysis and inflammation markers such as macrophages, transforming growth factor beta (TGF-beta) and inducible nitric oxide synthase (iNOS) expression; oxidative stress by plasma thiobarbituric acid reactive substances (TBARS); renal expression of angiotensin receptors subtype 1 (AT(1)) and 2 (AT(2)) and plasma concentration of angiotensin II. AIA rats showed elevated plasma levels of creatinine, urea, uric acid, TBARS and Ang II and reduced creatinine depuration, and enhanced kidney macrophage number, TGF-beta, caspase-3, iNOS and AT(1)/AT(2) receptors expression. The losartan reduced plasma creatinine and its clearance, reduced macrophages and the expression of TGF-beta and iNOS in renal tissues, and reduced plasma TBARS. We conclude that AIA causes kidney injury by a physiopathological mechanism that involves AT(1) stimulation in renal tissue, elevating the presence of macrophages, the expression of TGF-beta and iNOS, as well the local oxidative stress, which contribute to renal function deterioration. (AU)

• Processo FAPESP: 16/08450-3 - Influência da testosterona nas alterações vasculares promovidas pela artrite induzida por adjuvante que envolvem a angiotensina II
• Beneficiário: Agnaldo Bruno Chies
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/18730-6 - Avaliação da função renal e nível plasmático de TBARS em ratos artríticos
• Beneficiário: Rodrigo Piloto de Oliveira Batanero
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica