Microbial lipase: a new approach for a heterogeneous biocatalyst

Autor(es)

Tacin, Mariana Vendrasco ^{[1, 2]} ; Costa-Silva, Tales A. ^[3] ; de Paula, Ariela Veloso ^[1] ; Palomo, Jose M. ^[2] ; Santos-Ebinuma, Valeria de Carvalho ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Engn Bioproc & Biotechnol, Sch Pharmaceut Sci, Araraquara, SP - Brazil

^[2] Inst Catalysis ICP CSIC, Dept Biocatalysis, Canto Blanco - Spain

^[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharm, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 51, n. 8, p. 749-760, SEP 1 2021.
• Citações Web of Science: 2

Resumo

Lipases are enzymes employed in several industrial process and their applicability can be increased if these biocatalysts are in the immobilize form. The objective of this work was to study the immobilization of lipase produced by submerged cultivation of Aspergillus sp. by hydrophobic interaction, evaluating its stability and reuse capacity. The immobilization process on octyl-sepharose (C-8) and octadecyl-sepabeads (C-18) carriers was possible after the removal of oil excess presented in the fermented broth. The results showed that the enzyme was isolated and concentrated in octyl-sepharose with 22% of the initial activity. To increase the amount of enzyme adsorbed on the carrier, 4 immobilization cycles were performed in a row, on the same carrier, with a final immobilization yield of 151.32% and an increase in the specific activity of 136%. The activity test with immobilized lipase showed that the immobilized enzyme maintained 75% of the initial activity after 20 cycles. (AU)

• Processo FAPESP: 14/01580-3 - Processo biotecnológico visando o desenvolvimento de novos colorantes naturais microbianos para aplicação industrial
• Beneficiário: Valéria de Carvalho Santos Ebinuma
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/06908-8 - Desenvolvimento de nanomateriais sustentáveis para a purificação de fármacos antileucêmicos
• Beneficiário: Valéria de Carvalho Santos Ebinuma
• Modalidade de apoio: Auxílio à Pesquisa - Regular