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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial lipase: a new approach for a heterogeneous biocatalyst

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Author(s):
Tacin, Mariana Vendrasco [1, 2] ; Costa-Silva, Tales A. [3] ; de Paula, Ariela Veloso [1] ; Palomo, Jose M. [2] ; Santos-Ebinuma, Valeria de Carvalho [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Engn Bioproc & Biotechnol, Sch Pharmaceut Sci, Araraquara, SP - Brazil
[2] Inst Catalysis ICP CSIC, Dept Biocatalysis, Canto Blanco - Spain
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharm, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 51, n. 8, p. 749-760, SEP 1 2021.
Web of Science Citations: 2
Abstract

Lipases are enzymes employed in several industrial process and their applicability can be increased if these biocatalysts are in the immobilize form. The objective of this work was to study the immobilization of lipase produced by submerged cultivation of Aspergillus sp. by hydrophobic interaction, evaluating its stability and reuse capacity. The immobilization process on octyl-sepharose (C-8) and octadecyl-sepabeads (C-18) carriers was possible after the removal of oil excess presented in the fermented broth. The results showed that the enzyme was isolated and concentrated in octyl-sepharose with 22% of the initial activity. To increase the amount of enzyme adsorbed on the carrier, 4 immobilization cycles were performed in a row, on the same carrier, with a final immobilization yield of 151.32% and an increase in the specific activity of 136%. The activity test with immobilized lipase showed that the immobilized enzyme maintained 75% of the initial activity after 20 cycles. (AU)

FAPESP's process: 14/01580-3 - Biotechnological process for the development of new natural colorants from microorganisms for industrial application
Grantee:Valéria de Carvalho Santos Ebinuma
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/06908-8 - Development of sustainable nanomaterials for the purification of antileukemic drugs
Grantee:Valéria de Carvalho Santos Ebinuma
Support Opportunities: Regular Research Grants