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Biotechnological process for the development of new natural colorants from microorganisms for industrial application


In the last years, there has been a trend towards the replacement of synthetic colorants by natural ones, mainly due to the increase of consumer demand by more natural products. Thus, the research for new natural colorants, such as those produced by fungi, to be applied as bioadditives in a broad range of industrial sectors has been growing. In this context, it is important to optimize the production and extraction parameters, in order to reduce the overall cost production and enhance the entry of those bioproducts in the consumer market. This project purposes the study of all development steps of new natural colorants, namely, production, extraction, molecular identification and biological activity aiming their industrial application. In the first stage, it will be evaluated the colorants production in rotary shaker by submerged culture of Penicilllium purpurogenum DPUA 1275 employing alternative substracts (rice brain, wheat bran and sugar cane bagasse). After the optimization of the nutritional conditions using rotary shaker, it will be studied the colorants production in bioreactor, as well the adjustment of the stirring speed and aeration rate of bioreactor. In the purification stage, liquid-liquid extraction with organic solvents will be employed in order to isolate the coloring compounds. Then, the respective chemical structures will be determined using spectrophotometric techniques. Furthermore, it will be applied liquid-liquid extraction using aqueous two-phase systems (SABs) composed of Ionic Liquids (ILs) as an alternative technique for the purification and partition of natural colorants followed by counter-current chromatography to improve the purity of the extracted colorants. Finally, it will be also assessed the colorants biological activity. Considering the above, this proposal approaches different biotechnological aspects not only for production and extraction of microbial natural colorants but also their future applicability. (AU)

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Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, FERNANDA; HIRAI, PATRICIA RIE; SIMAS TEIXEIRA, MARIA FRANCISCA; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C. Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids. Separation and Purification Technology, v. 257, FEB 15 2021. Web of Science Citations: 1.
MUSSAGY, CASSAMO USSEMANE; CARIZIA GUIMARAES, ANA ALICE; FERREIRA ROCHA, LARA VICENTE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA DE CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Improvement of carotenoids production from Rhodotorula glutinis CCT-2186. Biochemical Engineering Journal, v. 165, JAN 15 2021. Web of Science Citations: 1.
DE OLIVEIRA, FERNANDA; LIMA, CAIO DE AZEVEDO; LOPES, ANDRE MORENI; VIANA MARQUES, DANIELA DE ARAUJO; DRUZIAN, JANICE IZABEL; PESSOA JUNIOR, ADALBERTO; SANTOS-EBINUMA, VALERIA CARVALHO. Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial. JOURNAL OF FUNGI, v. 6, n. 4 DEC 2020. Web of Science Citations: 0.
DE OLIVEIRA, FERNANDA; FERREIRA, LAURA CARMONA; BAPTISTA NETO, ALVARO; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS EBINUMA, VALERIA DE CARVALHO. Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor. Biochemical Engineering Journal, v. 161, SEP 15 2020. Web of Science Citations: 0.
MUSSAGY, CASSAMO U.; SANTOS-EBINUMA, VALERIA C.; GONZALEZ-MIQUEL, MARIA; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B. Protic Ionic Liquids as Cell-Disrupting Agents for the Recovery of Intracellular Carotenoids from Yeast Rhodotorula glutinis CCT-2186. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 19, p. 16765-16776, OCT 7 2019. Web of Science Citations: 0.
NASCIMENTO, PALOMA A. M.; PICHELI, FLAVIO P.; LOPES, ANDRE M.; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C. Effects of cholinium-based ionic liquids on Aspergillus niger lipase: Stabilizers or inhibitors. BIOTECHNOLOGY PROGRESS, v. 35, n. 5 SEP-OCT 2019. Web of Science Citations: 1.
DE OLIVEIRA, FERNANDA; PEDROLLI, DANIELLE BISCARO; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS-EBINUMA, VALERIA DE CARVALHO. Water-soluble fluorescent red colorant production by Talaromyces amestolkiae. Applied Microbiology and Biotechnology, v. 103, n. 16, p. 6529-6541, AUG 2019. Web of Science Citations: 0.
MUSSAGY, CASSAMO USSEMANE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Production and extraction of carotenoids produced by microorganisms. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1095-1114, FEB 2019. Web of Science Citations: 8.
ZACCARIM, BRUNA R.; DE OLIVEIRA, FERNANDA; PASSARINI, MICHEL R. Z.; DUARTE, ALYSSON W. F.; SETTE, LARA D.; JOZALA, ANGELA F.; TEIXEIRA, MARIA F. S.; SANTOS-EBINUMA, VALERIA DE CARVALHO. Sequencing and phylogenetic analyses of talaromyces amestolkiae from amazon: A producer of natural colorants. BIOTECHNOLOGY PROGRESS, v. 35, n. 1 JAN 2019. Web of Science Citations: 1.
DOS SANTOS, NATHALIA VIEIRA; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; BRANDAO PEREIRA, JORGE FERNANDO. Liquid-liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, SI, p. 1845-1863, JUL 2018. Web of Science Citations: 9.
ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, n. SI, p. 262-269, MAY 8 2018. Web of Science Citations: 2.
PANAS, PAWEL; LOPES, CAMILA; CERRI, MARCEL O.; VENTURA, SONIA P. M.; SANTOS-EBINUMA, VALERIA C.; PEREIRA, JORGE F. B. Purification of clavulanic acid produced by Streptomyces clavuligerus via submerged fermentation using polyethylene glycol/cholinium chloride aqueous two-phase systems. Fluid Phase Equilibria, v. 450, p. 42-50, OCT 25 2017. Web of Science Citations: 7.
ESTEVES TORRES, FABIO AURELIO; ZACCARIM, BRUNA REGINA; NOVAES, LETICIA CELIA DE LENCASTRE; JOZALA, ANGELA FAUSTINO; DOS SANTOS, CAROLINA ALVES; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS-EBINUMA, VALERIA CARVALHO. Natural colorants from filamentous fungi. Applied Microbiology and Biotechnology, v. 100, n. 6, p. 2511-2521, MAR 2016. Web of Science Citations: 14.

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