Biotechnological process for the development of new natural colorants from microorganisms for industrial application

• Grant number: 14/01580-3
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: January 01, 2015 - December 31, 2018
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Valéria de Carvalho Santos Ebinuma
• Grantee: Valéria de Carvalho Santos Ebinuma
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Adalberto Pessoa Junior ; André Gonzaga dos Santos ; Angela Faustino Jozala ; Edwil Aparecida de Lucca Gattas ; Jorge Pereira ; Sónia Patrícia Marques Ventura
• Associated grant(s): 21/06686-8 - Biotechnological process for the development of natural colorants from microbial sources for industrial application: phase II, AP.BIOEN.JP2; 16/23776-2 - Multi-User Equipment approved in grant 14/01580-3: counter current chromatography, AP.EMU
• Associated scholarship(s): 16/01076-9 - Purification of natural dyes obtained by filamentous fungi submerged culture using liquid-liquid extraction with organic solvents, BP.IC; 15/04751-6 - Extraction and chemical identification of natural colorants produce by Penicillium purpurogenum DPUA 1275, BP.DR

Abstract

In the last years, there has been a trend towards the replacement of synthetic colorants by natural ones, mainly due to the increase of consumer demand by more natural products. Thus, the research for new natural colorants, such as those produced by fungi, to be applied as bioadditives in a broad range of industrial sectors has been growing. In this context, it is important to optimize the production and extraction parameters, in order to reduce the overall cost production and enhance the entry of those bioproducts in the consumer market. This project purposes the study of all development steps of new natural colorants, namely, production, extraction, molecular identification and biological activity aiming their industrial application. In the first stage, it will be evaluated the colorants production in rotary shaker by submerged culture of Penicilllium purpurogenum DPUA 1275 employing alternative substracts (rice brain, wheat bran and sugar cane bagasse). After the optimization of the nutritional conditions using rotary shaker, it will be studied the colorants production in bioreactor, as well the adjustment of the stirring speed and aeration rate of bioreactor. In the purification stage, liquid-liquid extraction with organic solvents will be employed in order to isolate the coloring compounds. Then, the respective chemical structures will be determined using spectrophotometric techniques. Furthermore, it will be applied liquid-liquid extraction using aqueous two-phase systems (SABs) composed of Ionic Liquids (ILs) as an alternative technique for the purification and partition of natural colorants followed by counter-current chromatography to improve the purity of the extracted colorants. Finally, it will be also assessed the colorants biological activity. Considering the above, this proposal approaches different biotechnological aspects not only for production and extraction of microbial natural colorants but also their future applicability. (AU)

Scientific publications

(23)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, NATHALIA VIEIRA; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; BRANDAO PEREIRA, JORGE FERNANDO. Liquid-liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, SI, p. 1845-1863, JUL 2018. (14/16424-7, 14/01580-3, 14/19793-3, 13/08617-7, 16/07529-5)

DE OLIVEIRA, FERNANDA; FERREIRA, LAURA CARMONA; BAPTISTA NETO, ALVARO; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS EBINUMA, VALERIA DE CARVALHO. Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor. Biochemical Engineering Journal, v. 161, SEP 15 2020. (17/50303-0, 14/01580-3, 19/18076-0)

DE OLIVEIRA, FERNANDA; LIMA, CAIO DE AZEVEDO; LOPES, ANDRE MORENI; VIANA MARQUES, DANIELA DE ARAUJO; DRUZIAN, JANICE IZABEL; PESSOA JUNIOR, ADALBERTO; SANTOS-EBINUMA, VALERIA CARVALHO. Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (18/06908-8, 18/10799-0, 17/10789-1, 14/01580-3, 13/05039-2)

MUSSAGY, CASSAMO USSEMANE; CARIZIA GUIMARAES, ANA ALICE; FERREIRA ROCHA, LARA VICENTE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA DE CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Improvement of carotenoids production from Rhodotorula glutinis CCT-2186. Biochemical Engineering Journal, v. 165, JAN 15 2021. (17/50303-0, 14/16424-7, 14/01580-3, 14/19793-3, 19/09618-3)

MUSSAGY, CASSAMO U.; SANTOS-EBINUMA, VALERIA C.; GONZALEZ-MIQUEL, MARIA; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.. Protic Ionic Liquids as Cell-Disrupting Agents for the Recovery of Intracellular Carotenoids from Yeast Rhodotorula glutinis CCT-2186. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 19, p. 16765-16776, OCT 7 2019. (14/16424-7, 14/01580-3, 14/19793-3, 17/50303-0)

TACIN, MARIANA VENDRASCO; COSTA-SILVA, TALES A.; DE PAULA, ARIELA VELOSO; PALOMO, JOSE M.; SANTOS-EBINUMA, VALERIA DE CARVALHO. Microbial lipase: a new approach for a heterogeneous biocatalyst. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 51, n. 8, p. 749-760, SEP 1 2021. (14/01580-3, 18/06908-8)

ZACCARIM, BRUNA R.; DE OLIVEIRA, FERNANDA; PASSARINI, MICHEL R. Z.; DUARTE, ALYSSON W. F.; SETTE, LARA D.; JOZALA, ANGELA F.; TEIXEIRA, MARIA F. S.; SANTOS-EBINUMA, VALERIA DE CARVALHO. Sequencing and phylogenetic analyses of talaromyces amestolkiae from amazon: A producer of natural colorants. BIOTECHNOLOGY PROGRESS, v. 35, n. 1, JAN 2019. (14/01580-3)

MUSSAGY, CASSAMO USSEMANE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Production and extraction of carotenoids produced by microorganisms. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1095-1114, FEB 2019. (17/50303-0, 14/16424-7, 14/01580-3, 14/19793-3)

PANAS, PAWEL; LOPES, CAMILA; CERRI, MARCEL O.; VENTURA, SONIA P. M.; SANTOS-EBINUMA, VALERIA C.; PEREIRA, JORGE F. B.. Purification of clavulanic acid produced by Streptomyces clavuligerus via submerged fermentation using polyethylene glycol/cholinium chloride aqueous two-phase systems. Fluid Phase Equilibria, v. 450, p. 42-50, OCT 25 2017. (14/16424-7, 14/01580-3)

BARBIERI, GIORGIA S.; BENTO, HEITOR B. S.; DE OLIVEIRA, FERNANDA; PICHELI, FLAVIO P.; DIAS, LIDIA M.; MASARIN, FERNANDO; SANTOS-EBINUMA, VALERIA C.. Xylanase Production by Talaromyces amestolkiae Valuing Agroindustrial Byproducts. BIOTECH, v. 11, n. 2, p. 12-pg., 2022-05-17. (14/01580-3, 19/15493-9, 18/06908-8, 21/06686-8, 20/15513-7)

VERISSISMO, NATHALIA V.; NAKAMURA, CECILIA NAOMI; DE OLIVEIRA, FERNANDA; KUHN, BRUNA L.; FRIZZO, CLARISSA P.; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C.. Effect of amphiphilic ionic liquids on the colorimetric properties of polyketides colorants. JOURNAL OF MOLECULAR LIQUIDS, v. 363, p. 11-pg., 2022-07-30. (14/01580-3, 14/16424-7, 21/09175-4, 19/15493-9, 21/06686-8)

LIMA, CAIO A.; BENTO, HEITOR B. S.; PICHELI, FLAVIO P.; PAZ-CEDENO, FERNANDO R.; MUSSAGY, CASSAMO U.; MASARIN, FERNANDO; ACOSTA, MARIO A. TORRES; SANTOS-EBINUMA, VALERIA C.. Process development and techno-economic analysis of co- production of colorants and enzymes valuing agro-industrial citrus waste. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 35, p. 13-pg., 2023-10-01. (21/06686-8, 14/01580-3, 19/15493-9, 21/09175-4)

NASCIMENTO, PALOMA A. M.; PICHELI, FLAVIO P.; LOPES, ANDRE M.; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C.. Effects of cholinium-based ionic liquids on Aspergillus niger lipase: Stabilizers or inhibitors. BIOTECHNOLOGY PROGRESS, v. 35, n. 5, SEP-OCT 2019. (14/16424-7, 14/01580-3)

DOS REIS, BIANCA DALBEM; DE OLIVEIRA, FERNANDA; SANTOS-EBINUMA, VALERIA C.; FILLETTI, ERICA REGINA; NETO, ALVARO DE BAPTISTA. Assessment of artificial neural networks to predict red colorant production by Talaromyces amestolkiae. Bioprocess and Biosystems Engineering, v. 46, n. 1, p. 10-pg., 2022-11-28. (14/01580-3, 19/15493-9, 21/06686-8, 21/09175-4)

TACIN, MARIANA VENDRASCO; COSTA-SILVA, TALES A.; DE PAULA, ARIELA VELOSO; PALOMO, JOSE M.; SANTOS-EBINUMA, VALERIA DE CARVALHO. Microbial lipase: a new approach for a heterogeneous biocatalyst. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, NOV 2020. (18/06908-8, 14/01580-3)

ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, n. SI, p. 262-269, MAY 8 2018. (16/01076-9, 14/01580-3, 15/04751-6)

DE OLIVEIRA, FERNANDA; HIRAI, PATRICIA RIE; SIMAS TEIXEIRA, MARIA FRANCISCA; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C.. Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids. Separation and Purification Technology, v. 257, FEB 15 2021. (14/16424-7, 14/01580-3)

DE OLIVEIRA, FERNANDA; PEDROLLI, DANIELLE BISCARO; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS-EBINUMA, VALERIA DE CARVALHO. Water-soluble fluorescent red colorant production by Talaromyces amestolkiae. Applied Microbiology and Biotechnology, v. 103, n. 16, p. 6529-6541, AUG 2019. (14/01580-3)

ESTEVES TORRES, FABIO AURELIO; ZACCARIM, BRUNA REGINA; NOVAES, LETICIA CELIA DE LENCASTRE; JOZALA, ANGELA FAUSTINO; DOS SANTOS, CAROLINA ALVES; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS-EBINUMA, VALERIA CARVALHO. Natural colorants from filamentous fungi. Applied Microbiology and Biotechnology, v. 100, n. 6, p. 2511-2521, MAR 2016. (14/01580-3)

DE OLIVEIRA, FERNANDA; ZAPATA-BOADA, SANTIAGO; DA SILVA, S. S.; CUELLAR-FRANCA, ROSA M.; SANTOS-EBINUMA, VALERIA C.. Improving the Environmental Sustainability of Polyketides Colorants Production by Talaromyces Strain through Better Hydrodynamic Design in Bioreactors. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. N/A, p. 15-pg., 2022-10-17. (14/01580-3, 21/09175-4, 16/10636-8, 19/15493-9, 21/06686-8)

ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, p. 8-pg., 2018-05-08. (15/04751-6, 14/01580-3, 16/01076-9)

DOS SANTOS, NATHALIA VIEIRA; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; BRANDAO PEREIRA, JORGE FERNANDO. Liquid-liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, p. 19-pg., 2018-07-01. (14/16424-7, 14/01580-3, 16/07529-5, 13/08617-7, 14/19793-3)

DE OLIVEIRA, FERNANDA; ROCHA, INES L. D.; GOUVEIA ALVES PINTO, DIANA CLAUDIA; VENTURA, SONIA P. M.; DOS SANTOS, ANDRE GONZAGA; CREVELIN, EDUARDO JOSE; SANTOS EBINUMA, VALERIA DE CARVALHO. Identification of azaphilone derivatives of Monascus colorants from Talaromyces amestolkiae and their halochromic properties. Food Chemistry, v. 372, MAR 15 2022. (14/01580-3, 18/06908-8, 19/15493-9)