Purification of natural dyes obtained by filamentous fungi submerged culture using liquid-liquid extraction with organic solvents

• Grant number: 16/01076-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Valéria de Carvalho Santos Ebinuma
• Grantee: Ana Clara de Almeida Francisco
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 14/01580-3 - Biotechnological process for the development of new natural colorants from microorganisms for industrial application, AP.JP

Abstract

In the last few years with the increased demand by the population for a healthier life style and the growth of the natural colorants market in the world, there is a search for new natural colorants to be applied as bioadditives in several industries such as pharmaceutical , food and cosmetics. Natural colorants may be produced by animals, plants and microorganisms. Among the latter, filamentous fungi can be considered an alternative source for these compounds. In order to get these biocompounds without contaminants (pure) to identify their molecular structure is a fundamental step to evaluate the application into products on the market. The objective of this project is to purify natural coloring produced by submerged cultivation of Penicillium purpurogenum using liquid-liquid extraction. For this purpose, the colorants will be initially produced and after experiments with conventional liquid-liquid extraction using organic solvents of different polarities will be performed to purify the natural colorants present in the fermentation broth of P. purpurogenum DPUA 1275 and subsequently determine its chemical structure by spectrophotometric methods. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, n. SI, p. 262-269, MAY 8 2018. (16/01076-9, 14/01580-3, 15/04751-6)

ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, p. 8-pg., 2018-05-08. (15/04751-6, 14/01580-3, 16/01076-9)