Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions

Autor(es)

de Freitas, Emanuelle N. ^[1] ; Khatri, Vinay ^{[2, 3]} ; Wu, Jie ^[2] ; Takada, Masatsugu ^[2] ; Scarcella, Ana Silvia de A. ^[1] ; Martinez, Carlos Alberto ^[4] ; Saddler, Jack N. ^[2] ; Polizeli, Maria de Lourdes T. M. ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem & Immunol, FMRP, Sao Paulo - Brazil

^[2] Univ British Columbia, Fac Forestry, Dept Wood Sci, Vancouver, BC - Canada

^[3] Univ Quebec, Fac Sci, Dept Chem, SB-2540, President Kennedy Pavil, Montreal, PQ - Canada

^[4] Univ Sao Paulo, Dept Biol, FFCLRP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INDUSTRIAL CROPS AND PRODUCTS; v. 171, NOV 1 2021.
• Citações Web of Science: 0

Resumo

Panicum maximum represents an alternative as a dedicated energy crop for biorefinery. However, both pretreatment step and climate conditions impact its chemical composition and possibly its hydrolytic performance. Therefore, this study looks into integrating the future effect of the climate conditions and the pretreatment methods on the hydrolytic performance of P. maximum. Through a Trop-T-FACE system, P. maximum grown under three hypothetical future climate conditions: elevated temperature ((eT), which is 2 degrees C more than the ambient canopy temperature), elevated atmospheric CO2 concentration ((eC) up to 600 mu mol mol(-1)) and the combined effects (eT + eC). Subsequently, the groups were subjected to the liquid hot water (LHW) and organosolv (ORG) pretreatments, which enhanced biomass digestibility by mostly hemicellulose and lignin removal, respectively. Furthermore, optimized hydrolysis and fluorescent protein-tagged carbohydrate-binding modules binding suggested that P. maximum pretreated by LHW has greater saccharification yields and higher cellulose surface accessibility/exposure. This shows that hemicellulose directly impacts P. maximum recalcitrance, and its removal was the optimum pretreatment strategy. Concerning the studied expected future climate conditions, LHW samples did not show significant differences in hydrolysis yields at low solids loading (2%). However, at 10 % solids, eC had higher conversion yields (likely due to minimum end-product inhibition). Similarly, for organosolv pretreated samples, eC (at 2 and 10 % solid loading) exhibited greater cellulose-glucan conversion yields among the climate conditions groups and the highest cellulose accessibility. Thus, this work contributes to understand better the influence of both studied pretreatment methods and future climate conditions on the conversion efficiency of a critical energy crop and might assist in selecting the best pretreatment method to mitigate the climate effects and achieve greater hydrolysis yields. (AU)

• Processo FAPESP: 17/23989-9 - Biomassa lignocelulósica da gramínea forrageira Panicum maximum Jacq. produzida em condições simuladas de clima futuro: potencial fonte de açúcares fermentescíveis para bioenergia
• Beneficiário: Emanuelle Neiverth de Freitas
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/07522-6 - Desenvolvimento de tecnologias ecológicas para a hidrólise de biomassa e resíduos da indústria de celulose e papel
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/07141-5 - Formulação de um coquetel enzimático sinérgico otimizado com enzimas acessórias para melhorar o desempenho hidrolítico do Panicum maximum Jacq.
• Beneficiário: Emanuelle Neiverth de Freitas
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 08/58075-8 - Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species
• Beneficiário: Carlos Alberto Martinez y Huaman
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático