Strategies to Improve the Antimicrobial Efficacy of Photodynamic, Sonodynamic, and Sonophotodynamic Therapies

Autor(es)

Alves, Fernanda ^[1] ; Gomes Guimaraes, Gabriela ^[1] ; Mayumi Inada, Natalia ^[1] ; Pratavieira, Sebastiao ^[1] ; Salvador Bagnato, Vanderlei ^{[1, 2]} ; Kurachi, Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, Opt Grp, Ave Trabalhador Sao Carlense, 400 Ctr, BR-13560970 Sao Carlos, SP - Brazil

^[2] Texas A&M Univ, Coll Engn, Dept Biomed Engn, 101 Bizzell St, College Stn, TX 77843 - USA

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Surgery and Medicine; v. 53, n. 8, p. 1113-1121, OCT 2021.
• Citações Web of Science: 2

Resumo

Background and Objectives This work evaluated antimicrobial photodynamic therapy (PDT), sonodynamic therapy (SDT), and the association of both therapies (sonophotodynamic therapy {[}SPDT]), mediated by curcumin (Cur) against Staphylococcus aureus biofilm. Next, additional strategies for these treatments were assessed. Materials and Methods S. aureus biofilms received PDT, SDT, and SPDT, mediated by Cur (80 mu M), LED light (450 nm), and 1 MHz ultrasound. The same treatments were also performed adding a strategy: Cur with sodium dodecyl sulfate (SDS), Cur with potassium iodide (KI) or a pre-treatment with ultrasound. Cell viability was determined and biofilm architecture was evaluated under confocal microscopy. Results SPDT was more effective to inactivate the bacteria than PDT and SDT. SDS achieved the greatest viability reductions, followed by KI and ultrasound pre-treatment. Confocal images revealed biofilm disruption and a reduced number of cells in all treatments. However, SPDT exhibited a pronounced effect and it was greater using SDS. Conclusion SPDT was more effective and additional strategies potentiated its effectiveness. Lasers Surg. Med. (c) 2021 Wiley Periodicals LLC (AU)

• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs