Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo

Autor(es):Mostrar menos -

Bastos, Natalia Marins ^[1] ; Rossi, Guilherme Fazan ^[2] ; da Silva Leao, Beatriz Caetano ^[2] ; Negrao, Fernanda ^{[3, 4]} ; Ferreira, Christina Ramires ^{[5, 3]} ; Vrisman, Dayane Priscila ^[2] ; Rodrigues, Naiara Nantes ^[2] ; Zorzetto, Mariana Furtado ^[6] ; Freitas-Dell'Aqua, Camila De Paula ^[7] ; Vantini, Roberta ^[2] ; Monteiro, Fabio Morato ^{[2, 6]} ; Mercadante, Maria Eugenia Zerlotti ^[6] ; Eberlin, Marcos Nogueira ^[3] ; Hossepian de Lima, Vera Fernanda Martins ^[1] ; Mingoti, Gisele Zoccal ^{[8, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, Dept Anim Reprod, Grad Program Genet & Anim Breeding, Campus Jaboticabal, Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, Dept Anim Reprod, Grad Program Vet Med, Campus Jaboticabal, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[4] Northwestern Univ, Prote Ctr Excellence, 2170 Campus Dr, Evanston, IL - USA

^[5] Purdue Univ, Dept Chem, W Lafayette, IN 47907 - USA

^[6] Inst Zootecnia IZ APTA, Sertaozinho, SP - Brazil

^[7] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Campus Botucatu, Sao Paulo - Brazil

^[8] Sao Paulo State Univ UNESP, Sch Vet Med, Lab Reprod Physiol, Campus Aracatuba, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 175, p. 23-33, NOV 2021.
• Citações Web of Science: 0

Resumo

The aim of this study was to examine the effects of long-term dietary supplementation of young Nellore bulls with rumen-protected polyunsaturated fatty acids (PUFAs) and of the inclusion of catalase in the semen extender on semen quality, in vitro sperm fertilizing ability, and intracytoplasmic lipid content in the resulting embryos. Twelve Nellore bulls were supplemented with rumen-protected PUFAs or with a basal diet from 14 to 24 months of age. The semen was collected at the end of supplementation. For cryopreservation, the ejaculate was divided into two equal volumes and catalase was added to the extender in one of the fractions. Thus, the experimental design consisted of a 2 x 2 factorial scheme with two diets (control and PUFA) and two extenders (Cat thorn and Cat-). Total motility and the percentage of rapid cells in fresh semen were negatively affected by dietary supplementation with PUFAs (P < 0.05), but these effects did not persist after freezing. The frozen/thawed semen of animals fed PUFAs exhibited an increase in the percentages of damaged plasma and acrosomal membranes, as well as an increase in the proportion of lipids ions at m/z 578 and m/z 757 detected by MALDI-MS. Nevertheless, there was no effect of the treatments on in vitro embryo development. However, embryos derived from bulls supplemented with PUFAs exhibited higher lipid accumulation compared to control (P < 0.05). In conclusion, PUFA supplementation promoted worsening of semen quality without affecting the in vitro sperm fertilizing ability; however, the paternal diet affected the intracytoplasmic lipid content in the resulting embryos. (c) 2021 Published by Elsevier Inc. (AU)

• Processo FAPESP: 13/13696-3 - Parâmetros reprodutivos de machos da raça nelore de baixa e alta eficiência alimentar suplementados com ácidos graxosprotegidos em pastagem
• Beneficiário: Guilherme Fazan Rossi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06733-5 - Avaliação das características seminais em tourinhos da raça Nelore classificados para consumo alimentar residual (CAR) e suplementados com fonte de ácidos graxos poliinsaturados em regime de pastagem
• Beneficiário: Gisele Zoccal Mingoti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/11174-6 - Inclusão de exossomos derivados de fluido folicular durante a pré-maturação de oócitos bovinos com NPPC: efeitos sobre a maturação e a competência oocitária
• Beneficiário: Gisele Zoccal Mingoti
• Modalidade de apoio: Auxílio à Pesquisa - Regular