iological and molecular characterization of two closely related carlaviruses affecting brassica plant

Autor(es)

Oliveira, Agatha M. ^[1] ; Rodrigues, Leilane K. ^[1] ; Chabi-Jesus, Camila ^{[2, 3]} ; Chaves, Alexandre L. R. ^[1] ; Kitajima, Elliot W. ^[4] ; Harakava, Ricardo ^[3] ; Banguela-Castillo, Alexander ^[3] ; Ramos-Gonzalez, Pedro L. ^[3] ; Eiras, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Inst Biol, Ctr Pesquisa Sanidade Vegetal, Lab Fitovirol & Fisiopatol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, PPG Microbiol Agr, Piracicaba - Brazil

^[3] Inst Biol, ULR Biol Mol Aplicada, BR-04014900 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Nucleo Apoio Pesquisa Microscopia Eletron Aplicad, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT PATHOLOGY; v. 71, n. 2 SEP 2021.
• Citações Web of Science: 0

Resumo

Carlaviruses are plant-infecting viruses with flexuous filamentous particles of approximately 650 nm in length and a positive single-stranded hexacistronic RNA molecule as the genome. In this study, we analysed 14 samples of brassicas plants, reportedly affected by carlaviruses, that were collected in distant and edaphoclimatic distinct regions in Brazil. The genomes of four viral isolates detected in leaf kale (Brassica oleracea var. acephala) plants displayed the typical genomic organization of carlaviruses, subdivided into two contrasting identity profiles. Isolates T25, T107, and T110 showed a higher sequence identity among them (c.93%) than with T90 (c.67%), which had the highest nucleotide sequence identity (85.2%) with the only documented genomic fragment, approximately 1 kb of the RNA 3 `-end, of cole latent virus (CoLV). Identity values among the other three isolates and CoLV were consistently lower than 78.6%. Nucleotide and amino acid sequence identity values of the replicase cistron of the isolates T25 and T90 are below the threshold for species demarcation in the genus Carlavirus. Upon mechanical transmission, these two isolates induced different symptoms in brassicas and solanaceous plants. Overall, data indicated that these viruses belong to two related but distinct species of carlaviruses. While T90 was recognized as an isolate of CoLV, the remaining isolates were considered members of a new tentative carlavirus named Cole mild mosaic virus (CoMMV). Current work provides convincing support for the taxonomic status of the species Cole latent virus, enlarges the known diversity of carlaviruses, and makes available new molecular tools to improve surveys of brassica-infecting viruses. (AU)

• Processo FAPESP: 17/50334-3 - Plano de Desenvolvimento Institucional em Pesquisa (PDIp): modernização e adequação de unidades multiusuárias estratégicas do Instituto Biológico
• Beneficiário: Ana Eugênia de Carvalho Campos
• Modalidade de apoio: Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa
• Processo FAPESP: 17/18910-4 - Avaliação da diversidade dos patógenos moleculares (vírus e viróides de plantas) no Brasil: fase II-2018
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 18/17287-4 - Monitoramento da afidofauna em Brassica spp. e sua interação na dispersão de vírus em áreas de cultivo intensivo do estado de São Paulo
• Beneficiário: Alexandre Levi Rodrigues Chaves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22594-2 - Turnip mosaic vírus: levantamento, identificação, caracterização biológica e molecular, e aspectos epidemiológicos dos isolados brasileiros que infectam brássicas
• Beneficiário: Marcelo Eiras
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/50076-9 - Characterisation of Potyviruses infecting vegetable crops in Brazil and the stability of resistance identified in Europe to South American Potyviruses
• Beneficiário: Marcelo Eiras
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/06853-1 - Cole latent virus: caracterização biológica, molecular e epidemiológica
• Beneficiário: Agatha Mota de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado