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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

iological and molecular characterization of two closely related carlaviruses affecting brassica plant

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Author(s):
Oliveira, Agatha M. [1] ; Rodrigues, Leilane K. [1] ; Chabi-Jesus, Camila [2, 3] ; Chaves, Alexandre L. R. [1] ; Kitajima, Elliot W. [4] ; Harakava, Ricardo [3] ; Banguela-Castillo, Alexander [3] ; Ramos-Gonzalez, Pedro L. [3] ; Eiras, Marcelo [1]
Total Authors: 9
Affiliation:
[1] Inst Biol, Ctr Pesquisa Sanidade Vegetal, Lab Fitovirol & Fisiopatol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, PPG Microbiol Agr, Piracicaba - Brazil
[3] Inst Biol, ULR Biol Mol Aplicada, BR-04014900 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Nucleo Apoio Pesquisa Microscopia Eletron Aplicad, Piracicaba - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLANT PATHOLOGY; v. 71, n. 2 SEP 2021.
Web of Science Citations: 0
Abstract

Carlaviruses are plant-infecting viruses with flexuous filamentous particles of approximately 650 nm in length and a positive single-stranded hexacistronic RNA molecule as the genome. In this study, we analysed 14 samples of brassicas plants, reportedly affected by carlaviruses, that were collected in distant and edaphoclimatic distinct regions in Brazil. The genomes of four viral isolates detected in leaf kale (Brassica oleracea var. acephala) plants displayed the typical genomic organization of carlaviruses, subdivided into two contrasting identity profiles. Isolates T25, T107, and T110 showed a higher sequence identity among them (c.93%) than with T90 (c.67%), which had the highest nucleotide sequence identity (85.2%) with the only documented genomic fragment, approximately 1 kb of the RNA 3 `-end, of cole latent virus (CoLV). Identity values among the other three isolates and CoLV were consistently lower than 78.6%. Nucleotide and amino acid sequence identity values of the replicase cistron of the isolates T25 and T90 are below the threshold for species demarcation in the genus Carlavirus. Upon mechanical transmission, these two isolates induced different symptoms in brassicas and solanaceous plants. Overall, data indicated that these viruses belong to two related but distinct species of carlaviruses. While T90 was recognized as an isolate of CoLV, the remaining isolates were considered members of a new tentative carlavirus named Cole mild mosaic virus (CoMMV). Current work provides convincing support for the taxonomic status of the species Cole latent virus, enlarges the known diversity of carlaviruses, and makes available new molecular tools to improve surveys of brassica-infecting viruses. (AU)

FAPESP's process: 17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico
Grantee:Ana Eugênia de Carvalho Campos
Support Opportunities: Research Grants - State Research Institutes Modernization Program
FAPESP's process: 17/18910-4 - Diversity of molecular pathogens (viruses and viroids) present in Brazil. Phase II. 2018
Grantee:Elliot Watanabe Kitajima
Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 18/17287-4 - Monitoring of the aphid fauna in Brassica spp. and its interaction in the dispersion of viruses in intensive cropping in the State of São Paulo
Grantee:Alexandre Levi Rodrigues Chaves
Support Opportunities: Regular Research Grants
FAPESP's process: 14/22594-2 - Turnip mosaic virus: survey, identification, biological and molecular characterization, and epidemiological features of the Brazilian isolates that infect brassicas
Grantee:Marcelo Eiras
Support Opportunities: Regular Research Grants
FAPESP's process: 15/50076-9 - Characterisation of Potyviruses infecting vegetable crops in Brazil and the stability of resistance identified in Europe to South American Potyviruses
Grantee:Marcelo Eiras
Support Opportunities: Regular Research Grants
FAPESP's process: 19/06853-1 - Cole latent virus: biological, molecular and epidemiological characterization
Grantee:Agatha Mota de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate