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Cole latent virus: biological, molecular and epidemiological characterization

Grant number: 19/06853-1
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2019
Effective date (End): May 31, 2022
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Ricardo Harakava
Grantee:Agatha Mota de Oliveira
Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico, AP.PDIP


Cole Latent Virus (CoLV), genus Carlavirus, family Betaflexiviridae, has elongated flexuous particles of about 650 nm in length, and its genome consists of single-stranded RNA. This virus, which until now has only been described infecting brassica in Brazil, is transmitted mechanically and by aphids in a non-persistent manner. In the natural hosts, the CoLV infections are asymptomatic or induce vein clearing and mosaic on the leaves. Cole latent virus is considered a definitive species of the genus Carlavirus although the complete nucleotide sequence of the viral genome is unknown. As a consequence, the genetic variability of this virus as well as its epidemiology, and the interaction with other viruses common in brassicas, eg. Turnip Mosaic Virus (TuMV), remain ignored. Therefore, the objectives of this project are: (i) to evaluate the genetic diversity of CoLV isolates from different regions of Brazil through the complete sequencing of their genomes; (ii) to analyze aspects of its epidemiology by means of transmission tests with different species of aphids and challenging alternative hosts; (iii) to obtain purified CoLV preparations and produce polyclonal antiserum for use in PTA-ELSA and evaluate the use of this technique in field surveys; (iv) to develop primers for the detection of CoLV by means of RT-qPCR; (v) to estimate, by means of biological assays under controlled conditions, the effect of temperature on the expression of symptoms and on the development of varieties of brassicas inoculated with CoLV; (vi) to evaluate, under controlled conditions, the effects (synergistic or antagonistic) of CoLV co-infections with TuMV in brassica plants. We believe that the results of this project allow to increase knowledge of CoLV and the interaction with a potyvirus, TuMV, essential elements for the adoption of management strategies and control of the viruses in the field. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA, AGATHA M.; RODRIGUES, LEILANE K.; CHABI-JESUS, CAMILA; CHAVES, ALEXANDRE L. R.; KITAJIMA, ELLIOT W.; HARAKAVA, RICARDO; BANGUELA-CASTILLO, ALEXANDER; RAMOS-GONZALEZ, PEDRO L.; EIRAS, MARCELO. iological and molecular characterization of two closely related carlaviruses affecting brassica plant. PLANT PATHOLOGY, v. 71, n. 2, . (17/50334-3, 17/18910-4, 18/17287-4, 14/22594-2, 15/50076-9, 19/06853-1)

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