Swine viscera preservation in hypersaturated salt solution after alcohol fixation as a preparation method for educational purposes

Autor(es)

Guarana, Julia Belotto ^[1] ; Muller, Alois Foltran ^[1] ; Strefezzi, Ricardo de Francisco ^[1] ; Oliveira, Fabricio Singaretti ^[2] ; Machado, Luciana Cristina ^[1] ; Ambrosio, Carlos Eduardo ^[1] ; Sampaio Doria, Renata Gebara ^[1] ; de Freitas, Silvio Henrique ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Anim Morphol & Physiol, UNESP, Jaboticabal - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANATOMIA HISTOLOGIA EMBRYOLOGIA; v. 50, n. 6 SEP 2021.
• Citações Web of Science: 0

Resumo

The use of live animals for educational purposes is an old practice that is still employed in teaching and research institutions. However, there are several objections to this practice, whether for ethical or humanitarian reasons. Surgical techniques teaching using anatomical pieces and/or preserved cadavers promotes greater learning efficiency, provides exercise repetition and increases the confidence and satisfaction of the students when compared to the use of live animals. The current work aimed to analyse the feasibility of using fresh swine urinary bladder and small intestines (jejunum), obtained from slaughterhouses, fixed in 99.8% ethyl alcohol (EA) and preserved in sodium chloride hypersaturated solution (SCHS) at 30%, for 7, 14 and 21 days, as an alternative method for surgical skills training (SST). Swine viscera, fixed in EA and preserved in SCHS, presented a realistic appearance, absence of odour and maintained the viable morphological characteristics during the performance of the operative techniques. Preservation solutions had low cost, were easy to acquire and did not offers risks to human health. Therefore, urinary bladders and small intestines fixed in 99.8% EA for 30 days and maintained in 30% SCHS at different periods were demonstrated as a good viable option as a preservation method for surgical skills training. (AU)

• Processo FAPESP: 18/11026-4 - Preservação de vísceras de suínos em álcool etílico absoluto e solução hipertônica de cloreto de sódio como método alternativo para uso em aulas práticas da disciplina de técnica cirúrgica veterinária
• Beneficiário: Julia Belotto Guaraná
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica