Curaua-derived carbon dots: Fluorescent probes for effective Fe(III) ion detection, cellular labeling and bioimaging

Autor(es)

Raja, Sebastian ^{[1, 2, 3]} ; Buhl, Eva Miriam ^[4] ; Dreschers, Stephan ^[5] ; Schalla, Carmen ^{[2, 3]} ; Zenke, Martin ^{[2, 3]} ; Sechi, Antonio ^{[2, 3]} ; Mattoso, Luiz H. C. ^[1]

Afiliação do(s) autor(es)

^[1] EMBRAPA Instrumentacao, Natl Nanotechnol Lab Agribusiness LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, Pauwelsstr 30, D-52074 Aachen - Germany

^[3] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, Pauwelsstr 20, D-52074 Aachen - Germany

^[4] Rhein Westfal TH Aachen, Inst Pathol, Electron Microscopy Facil, Pauwelsstr 30, D-52074 Aachen - Germany

^[5] Rhein Westfal TH Aachen, Univ Hosp, Dept Pediat, Pauwelsstr 30, D-52074 Aachen - Germany

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 129, OCT 2021.
• Citações Web of Science: 0

Resumo

This study reports the generation of curau ` a-derived carbon dots (C-dots) and their suitability for Fe(III) detection, bioimaging and FACS analysis. C-dots were generated from curau ` a (Ananas erectifolius) fibers by a facile one-step hydrothermal approach. They exhibited graphite-like structure with a mean diameter of 2.4 nm, high water solubility, high levels of carboxyl and hydroxyl functional groups, excitation-dependent multicolor fluorescence emission (in the range 450 nm 560 nm) and superior photostability. C-dots were highly selective and effective for the detection of ferric Fe(III) ion in an aqueous medium with a detection limit of 0.77 mu M in the linear range of 0-30 mu M, a value much lower than the guideline limits proposed by the World Health Organization (WHO). In biological cell systems, C-dots were very well tolerated by B16F1 mouse melanoma and J774.A1 mouse macrophages cell lines, both of which effectively internalized C-dots in their cytoplasmic compartment. Finally, C dots were effective probes for long-term live cell imaging experiments and multi-channel flow cytometry analysis. Collectively, our findings demonstrate that curau ` a-derived C-dots serve as versatile and effective natural products for Fe(III) ion sensing, labeling and bioimaging of various cell types. This study adds novel C-dots to the library of carbon-based probes and paves the way towards a sustainable conversion of a most abundant biomass waste into value-added products. (AU)

• Processo FAPESP: 17/22017-3 - Nanocristais de celulose (NIR - CNC) de infravermelho próximo (NIR) para bioimagem celular
• Beneficiário: Raja Sebastian
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/00094-0 - Modificações químicas de nanowhiskers de celulose
• Beneficiário: Raja Sebastian
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado