Assessing different liquid-storage temperatures for rooster spermatozoa

Autor(es)

Blank, Marcel H. ^[1] ; Ruivo, Lourenco P. ^[1] ; Novaes, Gabriel A. ^[1] ; Lemos, Evelyn C. ^[1] ; Losano, Joao D. A. ^[2] ; Siqueira, Adriano F. P. ^[3] ; Pereira, Ricardo J. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Sci, Dept Anim Reprod, Grp Study Avian Multiplicat, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Sci, Dept Anim Reprod, Lab Androl, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Sci, Dept Anim Reprod, Lab Spermatozoa Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction Science; v. 233, OCT 2021.
• Citações Web of Science: 0

Resumo

Although liquid-storage is extensively used in poultry, there are still questions on how sperm physiology is affected and to what extent sperm functions are disrupted by storage temperature and time. There, therefore, was investigation of storage temperature and durations on multiple semen variables. The storage at 37 degrees C was the most damaging, affecting values for several variables within 4 h of storage, whereas most differences occurred between 5 and 25 degrees C after 8 h. Progressive motility and mitochondrial function started to decrease within 2 h at 25 and 37 degrees C, and within 4 h at 5 degrees C. Acrosomal damage only occurred in samples at 37 degrees C. Eosin-negrosin staining indicated there was damage to the plasma membrane at 37 degrees C, however, with use of propidium iodide there were differences between 5 and 25. C following 24 h. Temperatures of 5 and 25 degrees C resulted in similar curves for chromatin dispersion although chromatin integrities differed with storage for periods longer than 4 h. At 37 degrees C, results using both chromatin evaluations indicated there was damage after 2 h of incubation. Oxidative stress at 5 and 25 degrees C was similar when there was 24 h of storage. Intriguingly, there were no interaction between temperature and storage duration for peroxidized sperm membrane and total peroxidation status. These findings indicated that with a prolonged storage at 5 degrees C there were not marked changes in chicken spermatozoa, whereas at 25 degrees C there did not appear to be sperm damage occurring as a result of short-term storage. (AU)

• Processo FAPESP: 16/17158-4 - Conservação de germoplasma de cracídeos ameaçados por meio de transplantes interespecíficos de células germinativas
• Beneficiário: Ricardo Jose Garcia Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular