Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Peroxiredoxin AhpC1 protects Pseudomonas aeruginosa against the inflammatory oxidative burst and confers virulence

Texto completo
Autor(es):
Rocha, Leonardo Silva [1] ; da Silva, Beatriz Pereira [2] ; Correia, Thiago M. L. [3] ; da Silva, Railmara Pereira [2] ; Meireles, Diogo de Abreu [4] ; Pereira, Rafael [1, 3, 5] ; Soares Netto, Luis Eduardo [4] ; Meotti, Flavia Carla [2] ; Queiroz, Raphael Ferreira [1, 6]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Sudoeste Bahia, Programa Multicentr Posgrad Bioquim & Biol Mol, Vitoria Da Conquista, BA - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, Off 1001, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Fed Bahia, Inst Multidisciplinar Saude, Programa Multicentr Posgrad Multicentr Ciencias F, Salvador, BA - Brazil
[4] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil
[5] Univ Estadual Sudoeste Bahia, Dept Ciencias Biol, Vitoria Da Conquista, BA - Brazil
[6] Univ Estadual Sudoeste Bahia, Dept Ciencias Nat, Estr Bem Querer, Km 04 S-N, BR-45031900 Vitoria Da Conquista, BA - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: REDOX BIOLOGY; v. 46, OCT 2021.
Citações Web of Science: 0
Resumo

Pseudomonas aeruginosa is an opportunistic bacterium in patients with cystic fibrosis and hospital acquired infections. It presents a plethora of virulence factors and antioxidant enzymes that help to subvert the immune system. In this study, we identified the 2-Cys peroxiredoxin, alkyl-hydroperoxide reductase C1 (AhpC1), as a relevant scavenger of oxidants generated during inflammatory oxidative burst and a mechanism of P. aeruginosa (PA14) escaping from killing. Deletion of AhpC1 led to a higher sensitivity to hypochlorous acid (HOCl, IC50 3.2 +/- 0.3 versus 19.1 +/- 0.2 mu M), hydrogen peroxide (IC50 91.2 +/- 0.3 versus 496.5 +/- 6.4 mu M) and the organic peroxide urate hydroperoxide. Delta ahpC1 strain was more sensitive to the killing by isolated neutrophils and less virulent in a mice model of infection. All mice intranasally instilled with Delta ahpC1 survived as long as they were monitored (15 days), whereas 100% wild-type and Delta ahpC1 complemented with ahpC1 gene (Delta ahpC1 attB:ahpC1) died within 3 days. A significantly lower number of colonies was detected in the lung and spleen of Delta ahpC1-infected mice. Total leucocytes, neutrophils, myeloperoxidase activity, pro-inflammatory cytokines, nitrite production and lipid peroxidation were much lower in lungs or bronchoalveolar liquid of mice infected with Delta ahpC1. Purified AhpC neutralized the inflammatory organic peroxide, urate hydroperoxide, at a rate constant of 2.3 +/- 0.1 x 10(6) M(-1)s(-1), and only the Delta ahpC1 strain was sensitive to this oxidant. Incubation of neutrophils with uric acid, the urate hydroperoxide precursor, impaired neutrophil killing of wild-type but improved the killing of Delta ahpC1. Hyperuricemic mice presented higher levels of serum cytokines and succumbed much faster to PA14 infection when compared to normouricemic mice. In summary, Delta ahpC1 PA14 presented a lower virulence, which was attributed to a poorer ability to neutralize the oxidants generated by inflammatory oxidative burst, leading to a more efficient killing by the host. The enzyme is particularly relevant in detoxifying the newly reported inflammatory organic peroxide, urate hydroperoxide. (AU)

Processo FAPESP: 15/21563-9 - Estudo do papel do ácido úrico e da modulação redox sobre a atividade microbicida de células do sistema imune inato
Beneficiário:Railmara Pereira da Silva
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 15/10411-3 - EMU concedido no processo 2013/08166-5: stopped-flow
Beneficiário:Iolanda Midea Cuccovia
Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 18/14898-2 - Processos redox na inflamação e o seu papel sobre doenças inflamatórias
Beneficiário:Flavia Carla Meotti
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
Processo FAPESP: 19/26473-9 - Efeito do hydroperoxido de urato sobre a polarização de macrófagos
Beneficiário:Beatriz Pereira da Silva
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto