Load-matched acute and chronic exercise induce changes in mitochondrial biogenesis and metabolic markers

Autor(es)

Rodrigues, Natalia Almeida ^[1] ; Gobatto, Claudio Alexandre ^[1] ; Maia Forte, Lucas Dantas ^[1] ; de Barros Sousa, Filipe Antonio ^[2] ; Torsoni, Adriana Souza ^[3] ; de Fante, Thais ^[3] ; Manchado-Gobatto, Fulvia Barros ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Appl Sports Physiol, Limeira, SP - Brazil

^[2] Univ Fed Alagoas, Phys Educ & Sport Inst, Maceio, Alagoas - Brazil

^[3] Univ Estadual Campinas, Sch Appl Sci, Lab Metab Disorders, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 46, n. 10, p. 1196-1206, OCT 2021.
• Citações Web of Science: 0

Resumo

We investigated the effects of acute and chronic exercise, prescribed in different intensity zones, but with total load-matched on mitochondrial markers (cytochrome C oxidase subunit IV (COX-IV), mitochondrial transcription factor A (Tfam), and citrate synthase (CS) activity in skeletal muscles, heart, and liver), glycogen stores, aerobic capacity, and anaerobic index in swimming rats. For this, 2 experimental designs were performed (acute and chronic efforts). Load-matched exercises were prescribed below, above, and on the anaerobic threshold (AnT), determined by the lactate minimum test. In chronic programs, 2 training prescription strategies were assessed (monotonous and linear periodized model). Results show changes in glycogen stores but no modification in the COX-IV and Tfam contents after acute exercises. In the chronic protocols, COX-IV and Tfam proteins and CS adaptations were intensity- and tissue-dependent. Monotonous training promoted better adaptations than the periodized model. Training at 80% of the AnT improved both performance variables, emphasizing the anaerobic index, concomitant to CS and COX-IV improvement (soleus muscle). The aerobic capacity and CS activity (gastrocnemius) were increased after 120% AnT training. In conclusion, acute exercise protocol did not promote responses in mitochondrial target proteins. An intensity and tissue dependence were reported in the chronic protocols, highlighting training at 80 and 120% of the AnT. Novelty: Load-matched acute exercise did not enhance COX-IV and Tfam contents in skeletal muscles, heart, and liver. In chronic exercise, COX-IV, Tfam, and CS activity adaptations were intensity- and tissue-dependent. Monotonous training was more efficient than the periodized linear model in adaptations of target proteins and enzymatic activity. (AU)

• Processo FAPESP: 14/10336-9 - Efeito de diferentes modelos de treinamento com cargas controladas sobre marcadores fisiológicos, expressões gênicas e conteúdos protéicos da HIF-1 alfa, PGC-1 alfa, MCT1 e MCT4: relações com o rendimento e atividade espontânea de ratos nadadores
• Beneficiário: Fúlvia de Barros Manchado Gobatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/00272-6 - Efeitos da restrição calórica mediados pela SIRT3 sobre as capacidades aeróbia e anaeróbia de camundongos: relações com a atividade espontânea, parâmetros metabólicos e função mitocondrial muscular no envelhecimento
• Beneficiário: Fúlvia de Barros Manchado Gobatto
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 15/01362-9 - Efeitos da idade, dieta de restrição calórica (Cr) e SIRT3 sobre a atividade espontânea, mediadores comportamentais e oxidativos (NO, NOS e orexina) e respiração mitocondrial hipotalâmica
• Beneficiário: Claudio Alexandre Gobatto
• Modalidade de apoio: Bolsas no Exterior - Pesquisa