The influence of antineoplastic agents on the peri-implant bone around osseointegrated titanium implants: an in vivo histomorphometric and immunohistochemical study

Autor(es)

Matheus, Henrique Rinaldi ^{[1, 2]} ; Ervolino, Edilson ^{[3, 1]} ; Rodrigues Gusman, David Jonathan ^{[1, 2]} ; Forin, Luiz Guilherme ^{[1, 2]} ; Piovezan, Bianca Rafaeli ^{[1, 2]} ; de Almeida, Juliano Milanezi ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Dent, Nucleus Study & Res Periodont & Implantol NEPPI, Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Dept Diag & Surg, Periodont Div, St Jose Bonifacio 1193 Vila Mendonca, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; OCT 2021.
• Citações Web of Science: 0

Resumo

Background and objective The interaction between antineoplastic drugs used for treating cancer and non-affected tissues remains poorly assessed and may be critical for maintaining the quality of life for patients during and after treatment. This pre-clinical study evaluated the effects of cisplatin (CIS) and 5-fluorouracil (5-FU) on the peri-implant repair process around osseointegrated titanium implants installed in the tibiae of rats. Material and methods Were used 90 male rats, randomly divided into three groups (n = 30): physiological saline solution (PSS), CIS, and 5-FU. Titanium implants (4.0 x 2.2 mm) were inserted in both tibiae of all animals at day 0. The animals received either PSS, CIS, or 5-FU at 35 and 37 days. Euthanasia was performed at 50, 65, and 95 days after surgery. Histometric (bone/implant contact {[}BIC]) and bone area fraction occupancy (% BAFO), histological, and immunohistochemical (for bone morphogenetic protein 2/4 {[}BMP2/4], Runt-related transcription factor 2 {[}RUNX2], osteocalcin {[}OCN], and tartrate-resistant acid phosphatase {[}TRAP]) analyses were performed. Data were statistically analyzed. Results Groups CIS and 5-FU presented lower BIC and lower BAFO as compared with PSS in all time points. The imbalance in bone turnover was observed by the lower number of BMP2/4-, RUNX2-, and OCN-positive cells/mm(2) and the higher number of TRAP-positive cells/mm in groups CIS and 5-FU as compared with PSS in all time points. Persistent and exacerbated inflammation was observed in groups CIS and 5-FU. Conclusions Both antineoplastic agents interfered negatively in the bone turnover around osseointegrated titanium implants. (AU)

• Processo FAPESP: 17/11805-0 - Avaliação da influência da quimioterapia com cisplatina ou 5-Fluorouracil sobre o processo de reparo ósseo em implantes osseointegrados instalados em tíbias de ratos expostos ou não à nicotina
• Beneficiário: Henrique Rinaldi Matheus
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/11427-8 - Influência de drogas quimioterápicas na evolução da periodontite experimental em ratos
• Beneficiário: Juliano Milanezi de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular