Toxicity of alpha-Ag2WO4 microcrystals to freshwater microalga Raphidocelis subcapitata at cellular and population levels

Autor(es):Mostrar menos -

de Abreu, Cinthia Bruno ^{[1, 2]} ; Gebara, Renan Castelhano ^{[1, 2]} ; dos Reis, Larissa Luiza ^{[1, 2]} ; Rocha, Giseli Swerts ^[3] ; Goncalves Alho, Lays de Oliveira ^{[1, 2]} ; Alvarenga, Lais Mendes ^[4] ; Virtuoso, Luciano Sindra ^[4] ; Assis, Marcelo ^[5] ; Mansano, Adrislaine da Silva ^{[1, 2]} ; Longo, Elson ^[5] ; Gama Melao, Maria da Graca ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Dept Hydrobiol, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Fed Univ Sao Carlos UFSCar, Postgrad Program Ecol & Nat Resources PPGERN, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat NEEA CRHEA SHS, Ave Trabalhador Sao Carlense 400, BR-13560970 Sao Carlos, SP - Brazil

^[4] Fed Univ Alfenas UNIFAL MG, Chem Inst, Gabriel Monteiro Silva 700, BR-37130000 Alfenas, MG - Brazil

^[5] Fed Univ Sao Carlos UFSCar, Ctr Dev Funct Mat CDMF, POB 676, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 288, n. 2 FEB 2022.
• Citações Web of Science: 0

Resumo

Silver-based materials have microbicidal action, photocatalytic activity and electronic properties. The increase in manufacturing and consumption of these compounds, given their wide functionality and application, is a source of contamination to freshwater ecosystems and causes toxicity to aquatic biota. Therefore, for the first time, we evaluated the toxicity of the silver tungstate (alpha-Ag2WO4), in different morphologies (cube and rod), for the microalga Raphidocelis subcapitata. To investigate the toxicity, we evaluated the growth rate, cell complexity and size, reactive oxygen species (ROS) production and chlorophyll a (Chl a) fluorescence. The alpha-Ag2WO4 - R (rod) was 1.7 times more toxic than alpha-Ag2WO4-C (cube), with IC10 and IC50 values of, respectively, 8.68 +/- 0.91 mu g L-1 and 13.72 +/- 1.48 mu g L-1 for alpha-Ag2WO4 - R and 18.60 +/- 1.61 mu g L-1 and 23.47 +/- 1.16 mu g L-1 for alpha-Ag2WO4-C. The release of silver ions was quantified and indicated that the silver ions dissolution from the alpha-Ag2WO4 - R ranged from 34 to 71%, while the Ag ions from the alpha-Ag2WO4-C varied from 35 to 97%. The alpha-Ag2WO4-C induced, after 24 h exposure, the increase of ROS at the lowest concentrations (8.81 and 19.32 mu g L-1), whereas the alpha-Ag2WO4 - R significantly induced ROS production at 96 hat the highest concentration (31.76 mu g L-1). Both microcrystal shapes significantly altered the cellular complexity and decreased the Chl a fluorescence at all tested concentrations. We conclude that the different morphologies of alpha-Ag2WO4 negatively affect the microalga and are important sources of silver ions leading to harmful consequences to the aquatic ecosystem. (AU)

• Processo FAPESP: 18/07988-5 - Bioprospecção, caracterização e otimização de microalgas brasileiras para a biofixação de CO2 e produção de biomoléculas de importância comercial
• Beneficiário: Ana Teresa Lombardi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs