emi-continuous Cultivation of Chlorella minutissima in Landfill Leachate: Effect of Process Variables on Biomass Compositio

Autor(es)

dos Santos, Wallyson Ribeiro ^[1] ; Tagliaferro, Geronimo Virginio ^[1] ; dos Santos, Julio Cesar ^[2] ; Pereira, Priscila ^[1] ; Roma, Carolina ^[1] ; Silva, Messias Borges ^[1] ; Pelegrine Guimaraes, Daniela Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Chem Engn, Lorena, SP - Brazil

^[2] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, Lorena, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WASTE AND BIOMASS VALORIZATION; v. 13, n. 3, SI OCT 2021.
• Citações Web of Science: 0

Resumo

Purpose Microalgal biomass composition can be modulated by changing the type of bioreactor and cultivation conditions. Besides, these microorganisms can produce valuable biomass components from unconventional culture media, including effluents and wastes. Our objective was to evaluate the production and composition of biomass in an airlift bioreactor operated in a semi-continuous mode to cultivate the microalgae Chlorella minutissima in a landfill leachate-based medium. Methods Chlorella minutissima was cultivated in an airlift bioreactor operated in semi-continuous mode, applying a Taguchi L9 orthogonal arrangement and analyzing the effects of factors landfill leachate concentration (LC), percentage flux of CO2 added to the gas stream (GP), aeration flow in the reactor (AF), and feed flow (FR), on the cellular composition of biomass. Results The microalgae produced high protein levels, reaching a maximum content of 69.60% (LC: 10%, GP: null, AF: 0.30 vvm and FR: 0.9 mu(max)). The highest contents of lipid and carbohydrate were 17.4% (LC: 15%, GP: null, AF: 0.45 vvm and FR: 0.7 mu(max) vvm) and 11.6% (LC: 10%, GP: 15%, AF: 0.15 vvm and FR: 0.7 mu(max)). Statistical analysis showed LC was the most influential factor. GP did not show significance for any response, while the variables AF and FR showed significance in the productivity of proteins. Conclusion The use of unconventional cultivation media in airlift bioreactor operated in semi-continuous mode was shown as a system with potential to be used to valorize leachate by producing valuable biomass, allowing to obtain different cell composition by varying the process conditions. (AU)

• Processo FAPESP: 16/23416-6 - Análise dos efeitos da configuração do biorreator e das variáveis de processo nos parâmetros reológicos da biomassa da Chlorella sp ao longo das diversas etapas para a produção dos biocombustíveis
• Beneficiário: Daniela Helena Pelegrine Guimarães
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 14/03244-0 - Análise do comportamento reológico de suspensões de Chlorella sp. ao longo das diversas etapas para a produção do biodiesel
• Beneficiário: Daniela Helena Pelegrine Guimarães
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular