ging whole blood transcriptome reveals candidate genes for SARS-CoV-2-related vascular and immune alteration

Autor(es)

de Almeida Chuffa, Luiz Gustavo ^[1] ; Freire, Paula Paccielli ^[2] ; Souza, Jeferson dos Santos ^[1] ; de Mello, Mariana Costa ^[3] ; Neto, Mario de Oliveira ^[3] ; Carvalho, Robson Francisco ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, 510 Rubiao Jr, S-N, POB, BR-18618689 Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[3] UNESP Sao Paulo State Univ, Inst Biosci, Dept Biophys & Pharmacol, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MOLECULAR MEDICINE-JMM; v. 100, n. 2 NOV 2021.
• Citações Web of Science: 1

Resumo

The risk of severe COVID-19 increases with age as older patients are at highest risk. Thus, there is an urgent need to identify how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts with blood components during aging. We investigated the whole blood transcriptome from the Genotype-Tissue Expression (GTEx) database to explore differentially expressed genes (DEGs) translated into proteins interacting with viral proteins during aging. From 22 DEGs in aged blood, FASLG, CTSW, CTSE, VCAM1, and BAG3 were associated with immune response, inflammation, cell component and adhesion, and platelet activation/aggregation. Males and females older than 50 years old overexpress FASLG, possibly inducing a hyperinflammatory cascade. The expression of cathepsins (CTSW and CTSE) and the anti-apoptotic co-chaperone molecule BAG3 also increased throughout aging in both genders. By exploring single-cell RNA-sequencing data from peripheral blood of SARS-CoV-2-infected patients, we found FASLG and CTSW expressed in natural killer cells and CD8 + T lymphocytes, whereas BAG3 was expressed mainly in CD4 + T cells, naive T cells, and CD14 + monocytes. In addition, T cell exhaustion was associated with increased expression of CCL4L2 and DUSP4 over blood aging. LAG3, PDCD1, TIGIT, VCAM1, HLA-DRA, and TOX also increased in individuals aged 60-69 years old; conversely, the RGS2 gene decreased with aging. We further identified a distinct gene expression profile associated with type I interferon signaling following blood aging. These results revealed changes in blood molecules potentially related to SARS-CoV-2 infection throughout aging, emphasizing them as therapeutic candidates for aggressive clinical manifestation of COVID-19. Key messages center dot Prediction of host-viral interactions in the whole blood transcriptome during aging. center dot Expression levels of FASLG, CTSW, CTSE, VCAM1, and BAG3 increase in aged blood. center dot Blood interactome reveals targets involved with immune response, inflammation, and blood clots. center dot SARS-CoV-2-infected patients with high viral load showed FASLG overexpression. center dot Gene expression profile associated with T cell exhaustion and type I interferon signaling were affected with blood aging. (AU)

• Processo FAPESP: 19/00906-6 - Melatonina e seus receptores MT1 e MT2: efeitos na apoptose, proliferação e potencial migratório de células de carcinoma ovariano (linhagem SKOV-3)
• Beneficiário: Luiz Gustavo de Almeida Chuffa
• Modalidade de apoio: Auxílio à Pesquisa - Regular